Xenopus oocyte as a model system to study store-operated Ca 2+ entry (SOCE)

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2 Citations (Scopus)

Abstract

Store-operated Ca 2+ entry (SOCE) is a ubiquitous Ca 2+ influx pathway at the cell membrane that is regulated by Ca 2+ content in intracellular stores. SOCE is important for a multitude of physiological processes, including muscle development, T-cell activation, and fertilization. Therefore, understanding the molecular regulation of SOCE is imperative. SOCE activation requires conformational and spatial changes in proteins located in both the endoplasmic reticulum and plasma membrane. This leads to the generation of an ionic current of very small amplitude. Both biochemical and electrophysiological parameters of SOCE can be difficult to record in small mammalian cells. In this protocol we present the different methodologies that enable the study of SOCE in a unique model system, the frog oocyte, which provides several advantages and have contributed significantly to our understanding of SOCE regulation.

Original languageEnglish
Article number66
JournalFrontiers in Cell and Developmental Biology
Volume4
Issue numberJUN
DOIs
Publication statusPublished - 24 Jun 2016

Fingerprint

Xenopus
Oocytes
Cell Membrane
Physiological Phenomena
Muscle Development
Fertilization
Endoplasmic Reticulum
Anura
T-Lymphocytes
Proteins

Keywords

  • Confocal imaging
  • Meiosis
  • RNA injection
  • Store-operated Ca entry (SOCE)
  • Two-electrode voltage clamp
  • Xenopus oocyte

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

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abstract = "Store-operated Ca 2+ entry (SOCE) is a ubiquitous Ca 2+ influx pathway at the cell membrane that is regulated by Ca 2+ content in intracellular stores. SOCE is important for a multitude of physiological processes, including muscle development, T-cell activation, and fertilization. Therefore, understanding the molecular regulation of SOCE is imperative. SOCE activation requires conformational and spatial changes in proteins located in both the endoplasmic reticulum and plasma membrane. This leads to the generation of an ionic current of very small amplitude. Both biochemical and electrophysiological parameters of SOCE can be difficult to record in small mammalian cells. In this protocol we present the different methodologies that enable the study of SOCE in a unique model system, the frog oocyte, which provides several advantages and have contributed significantly to our understanding of SOCE regulation.",
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