Wide-field assessment of the human corneal subbasal nerve plexus in diabetic neuropathy using a novel mapping technique

Katie Edwards, Nicola Pritchard, Kevin Gosschalk, Geoff P. Sampson, Anthony Russell, Rayaz Malik, Nathan Efron

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

To develop a rapid optimized technique of wide-field imaging of the human corneal subbasal nerve plexus. A dynamic fixation target was developed and, coupled with semiautomated tiling software, a rapid method of capturing and montaging multiple corneal confocal microscopy images was created. To illustrate the utility of this technique, wide-field maps of the subbasal nerve plexus were produced in 2 participants with diabetes, 1 with and 1 without neuropathy. The technique produced montages of the central 3 mm of the subbasal corneal nerve plexus. The maps seem to show a general reduction in the number of nerve fibers and branches in the diabetic participant with neuropathy compared with the individual without neuropathy. This novel technique will allow more routine and widespread use of subbasal nerve plexus mapping in clinical and research situations. The significant reduction in the time to image the corneal subbasal nerve plexus should expedite studies of larger groups of diabetic patients and those with other conditions affecting nerve fibers. The inferior whorl and the surrounding areas may show the greatest loss of nerve fibers in individuals with diabetic neuropathy, but this should be further investigated in a larger cohort.

Original languageEnglish
Pages (from-to)1078-1082
Number of pages5
JournalCornea
Volume31
Issue number9
DOIs
Publication statusPublished - Sep 2012
Externally publishedYes

Fingerprint

Diabetic Neuropathies
Nerve Fibers
Type 1 Diabetes Mellitus
Confocal Microscopy
Software
Research

Keywords

  • cornea
  • corneal nerves
  • diabetes
  • diabetic neuropathy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Wide-field assessment of the human corneal subbasal nerve plexus in diabetic neuropathy using a novel mapping technique. / Edwards, Katie; Pritchard, Nicola; Gosschalk, Kevin; Sampson, Geoff P.; Russell, Anthony; Malik, Rayaz; Efron, Nathan.

In: Cornea, Vol. 31, No. 9, 09.2012, p. 1078-1082.

Research output: Contribution to journalArticle

Edwards, Katie ; Pritchard, Nicola ; Gosschalk, Kevin ; Sampson, Geoff P. ; Russell, Anthony ; Malik, Rayaz ; Efron, Nathan. / Wide-field assessment of the human corneal subbasal nerve plexus in diabetic neuropathy using a novel mapping technique. In: Cornea. 2012 ; Vol. 31, No. 9. pp. 1078-1082.
@article{18ceaf87132a400a99f2846690e9ab7e,
title = "Wide-field assessment of the human corneal subbasal nerve plexus in diabetic neuropathy using a novel mapping technique",
abstract = "To develop a rapid optimized technique of wide-field imaging of the human corneal subbasal nerve plexus. A dynamic fixation target was developed and, coupled with semiautomated tiling software, a rapid method of capturing and montaging multiple corneal confocal microscopy images was created. To illustrate the utility of this technique, wide-field maps of the subbasal nerve plexus were produced in 2 participants with diabetes, 1 with and 1 without neuropathy. The technique produced montages of the central 3 mm of the subbasal corneal nerve plexus. The maps seem to show a general reduction in the number of nerve fibers and branches in the diabetic participant with neuropathy compared with the individual without neuropathy. This novel technique will allow more routine and widespread use of subbasal nerve plexus mapping in clinical and research situations. The significant reduction in the time to image the corneal subbasal nerve plexus should expedite studies of larger groups of diabetic patients and those with other conditions affecting nerve fibers. The inferior whorl and the surrounding areas may show the greatest loss of nerve fibers in individuals with diabetic neuropathy, but this should be further investigated in a larger cohort.",
keywords = "cornea, corneal nerves, diabetes, diabetic neuropathy",
author = "Katie Edwards and Nicola Pritchard and Kevin Gosschalk and Sampson, {Geoff P.} and Anthony Russell and Rayaz Malik and Nathan Efron",
year = "2012",
month = "9",
doi = "10.1097/ICO.0b013e318245c012",
language = "English",
volume = "31",
pages = "1078--1082",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - Wide-field assessment of the human corneal subbasal nerve plexus in diabetic neuropathy using a novel mapping technique

AU - Edwards, Katie

AU - Pritchard, Nicola

AU - Gosschalk, Kevin

AU - Sampson, Geoff P.

AU - Russell, Anthony

AU - Malik, Rayaz

AU - Efron, Nathan

PY - 2012/9

Y1 - 2012/9

N2 - To develop a rapid optimized technique of wide-field imaging of the human corneal subbasal nerve plexus. A dynamic fixation target was developed and, coupled with semiautomated tiling software, a rapid method of capturing and montaging multiple corneal confocal microscopy images was created. To illustrate the utility of this technique, wide-field maps of the subbasal nerve plexus were produced in 2 participants with diabetes, 1 with and 1 without neuropathy. The technique produced montages of the central 3 mm of the subbasal corneal nerve plexus. The maps seem to show a general reduction in the number of nerve fibers and branches in the diabetic participant with neuropathy compared with the individual without neuropathy. This novel technique will allow more routine and widespread use of subbasal nerve plexus mapping in clinical and research situations. The significant reduction in the time to image the corneal subbasal nerve plexus should expedite studies of larger groups of diabetic patients and those with other conditions affecting nerve fibers. The inferior whorl and the surrounding areas may show the greatest loss of nerve fibers in individuals with diabetic neuropathy, but this should be further investigated in a larger cohort.

AB - To develop a rapid optimized technique of wide-field imaging of the human corneal subbasal nerve plexus. A dynamic fixation target was developed and, coupled with semiautomated tiling software, a rapid method of capturing and montaging multiple corneal confocal microscopy images was created. To illustrate the utility of this technique, wide-field maps of the subbasal nerve plexus were produced in 2 participants with diabetes, 1 with and 1 without neuropathy. The technique produced montages of the central 3 mm of the subbasal corneal nerve plexus. The maps seem to show a general reduction in the number of nerve fibers and branches in the diabetic participant with neuropathy compared with the individual without neuropathy. This novel technique will allow more routine and widespread use of subbasal nerve plexus mapping in clinical and research situations. The significant reduction in the time to image the corneal subbasal nerve plexus should expedite studies of larger groups of diabetic patients and those with other conditions affecting nerve fibers. The inferior whorl and the surrounding areas may show the greatest loss of nerve fibers in individuals with diabetic neuropathy, but this should be further investigated in a larger cohort.

KW - cornea

KW - corneal nerves

KW - diabetes

KW - diabetic neuropathy

UR - http://www.scopus.com/inward/record.url?scp=84865446451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865446451&partnerID=8YFLogxK

U2 - 10.1097/ICO.0b013e318245c012

DO - 10.1097/ICO.0b013e318245c012

M3 - Article

VL - 31

SP - 1078

EP - 1082

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 9

ER -