Wet chemistry and peptide immobilization on polytetrafluoroethylene for improved cell-adhesion

Matthias Gabriel, Kerstin Niederer, Holger Frey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Endowing materials surface with cell-adhesive properties is a common strategy in biomaterial research and tissue engineering. This is particularly interesting for already approved polymers that have a long standing use in medicine because these materials are well characterized and legal issues associated with the introduction of newly synthesized polymers may be avoided. Polytetrafluoroethylene (PTFE) is one of the most frequently employed materials for the manufacturing of vascular grafts but the polymer lacks cell adhesion promoting features. Endothelialization, i.e., complete coverage of the grafts inner surface with a confluent layer of endothelial cells is regarded key to optimal performance, mainly by reducing thrombogenicity of the artificial interface. This study investigates the growth of endothelial cells on peptide-modified PTFE and compares these results to those obtained on unmodified substrate. Coupling with the endothelial cell adhesive peptide Arg-Glu-Asp-Val (REDV) is performed via activation of the fluorin-containing polymer using the reagent sodium naphthalenide, followed by subsequent conjugation steps. Cell culture is accomplished using Human Umbilical Vein Endothelial Cells (HUVECs) and excellent cellular growth on peptide-immobilized material is demonstrated over a two-week period.

Original languageEnglish
Article numbere54272
JournalJournal of Visualized Experiments
Volume2016
Issue number114
DOIs
Publication statusPublished - 15 Aug 2016
Externally publishedYes

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Keywords

  • Bioengineering
  • Biomaterial
  • Blood contacting surfaces
  • Endothelial cell
  • Endothelialization
  • Issue 114
  • Peptide immobilization
  • Polytetrafluoroethylene (PTFE)
  • REDV
  • Surface modification
  • Tissue engineering
  • Vascular graft

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

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