Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome

Flore Mietton, Aditya K. Sengupta, Annie Molla, Gisele Picchi, Sophie Barral, Laurent Heliot, Thierry Grange, Anton Wutz, Stefan Dimitrov

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains ∼1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1- immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalMolecular and Cellular Biology
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

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X Chromosome
Histones
Green Fluorescent Proteins
Chromosomes
Chromosome Structures
Chromosomal Instability
Nucleosomes
Genomic Instability
Chromatin Immunoprecipitation
HEK293 Cells
Antibodies
Liver
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome. / Mietton, Flore; Sengupta, Aditya K.; Molla, Annie; Picchi, Gisele; Barral, Sophie; Heliot, Laurent; Grange, Thierry; Wutz, Anton; Dimitrov, Stefan.

In: Molecular and Cellular Biology, Vol. 29, No. 1, 01.2009, p. 150-156.

Research output: Contribution to journalArticle

Mietton, F, Sengupta, AK, Molla, A, Picchi, G, Barral, S, Heliot, L, Grange, T, Wutz, A & Dimitrov, S 2009, 'Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome', Molecular and Cellular Biology, vol. 29, no. 1, pp. 150-156. https://doi.org/10.1128/MCB.00997-08
Mietton, Flore ; Sengupta, Aditya K. ; Molla, Annie ; Picchi, Gisele ; Barral, Sophie ; Heliot, Laurent ; Grange, Thierry ; Wutz, Anton ; Dimitrov, Stefan. / Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome. In: Molecular and Cellular Biology. 2009 ; Vol. 29, No. 1. pp. 150-156.
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