Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome

Flore Mietton, Aditya K. Sengupta, Annie Molla, Gisele Picchi, Sophie Barral, Laurent Heliot, Thierry Grange, Anton Wutz, Stefan Dimitrov

Research output: Contribution to journalArticle

42 Citations (Scopus)


We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains ∼1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1- immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalMolecular and Cellular Biology
Issue number1
Publication statusPublished - 1 Jan 2009


ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Mietton, F., Sengupta, A. K., Molla, A., Picchi, G., Barral, S., Heliot, L., Grange, T., Wutz, A., & Dimitrov, S. (2009). Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome. Molecular and Cellular Biology, 29(1), 150-156. https://doi.org/10.1128/MCB.00997-08