Waterpipe smoking induces epigenetic changes in the small airway epithelium

Matthew S. Walters, Jacqueline Salit, Jin Hyun Ju, Michelle R. Staudt, Robert J. Kaner, Allison M. Rogalski, Teniola B. Sodeinde, Riyaad Rahim, Yael Strulovici-Barel, Jason G. Mezey, Ahmad M. Almulla, Hisham Sattar, Mai Mahmoud, Ronald Crystal

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Waterpipe (also called hookah, shisha, or narghile) smoking is a common form of tobacco use in the Middle East. Its use is becoming more prevalent in Western societies, especially among young adults as an alternative form of tobacco use to traditional cigarettes. While the risk to cigarette smoking is well documented, the risk to waterpipe smoking is not well defined with limited information on its health impact at the epidemiologic, clinical and biologic levels with respect to lung disease. Based on the knowledge that airway epithelial cell DNA methylation is modified in response to cigarette smoke and in cigarette smoking-related lung diseases, we assessed the impact of light-use waterpipe smoking on DNA methylation of the small airway epithelium (SAE) and whether changes in methylation were linked to the transcriptional output of the cells. Small airway epithelium was obtained from 7 nonsmokers and 7 light-use (2.6 ± 1.7 sessions/wk) waterpipe-only smokers. Genome-wide comparison of SAE DNA methylation of waterpipe smokers to nonsmokers identified 727 probesets differentially methylated (fold-change >1.5, p<0.05) representing 673 unique genes. Dominant pathways associated with these epigenetic changes include those linked to G-protein coupled receptor signaling, aryl hydrocarbon receptor signaling and xenobiotic metabolism signaling, all of which have been associated with cigarette smoking and lung disease. Of the genes differentially methylated, 11.3% exhibited a corresponding significant (p<0.05) change in gene expression with enrichment in pathways related to regulation of mRNA translation and protein synthesis (eIF2 signaling and regulation of eIF4 and p70S6K signaling). Overall, these data demonstrate that light-use waterpipe smoking is associated with epigenetic changes and related transcriptional modifications in the SAE, the cell population demonstrating the earliest pathologic abnormalities associated with chronic cigarette smoking.

Original languageEnglish
Article numbere0171112
JournalPLoS One
Volume12
Issue number3
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Fingerprint

smoking (habit)
Epigenomics
epigenetics
Tobacco Products
DNA methylation
epithelium
Epithelium
Smoking
tobacco use
respiratory tract diseases
Pulmonary diseases
cigarettes
DNA Methylation
Tobacco
Genes
Lung Diseases
Tobacco Use
Middle East
smoke
xenobiotics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Walters, M. S., Salit, J., Ju, J. H., Staudt, M. R., Kaner, R. J., Rogalski, A. M., ... Crystal, R. (2017). Waterpipe smoking induces epigenetic changes in the small airway epithelium. PLoS One, 12(3), [e0171112]. https://doi.org/10.1371/journal.pone.0171112

Waterpipe smoking induces epigenetic changes in the small airway epithelium. / Walters, Matthew S.; Salit, Jacqueline; Ju, Jin Hyun; Staudt, Michelle R.; Kaner, Robert J.; Rogalski, Allison M.; Sodeinde, Teniola B.; Rahim, Riyaad; Strulovici-Barel, Yael; Mezey, Jason G.; Almulla, Ahmad M.; Sattar, Hisham; Mahmoud, Mai; Crystal, Ronald.

In: PLoS One, Vol. 12, No. 3, e0171112, 01.03.2017.

Research output: Contribution to journalArticle

Walters, MS, Salit, J, Ju, JH, Staudt, MR, Kaner, RJ, Rogalski, AM, Sodeinde, TB, Rahim, R, Strulovici-Barel, Y, Mezey, JG, Almulla, AM, Sattar, H, Mahmoud, M & Crystal, R 2017, 'Waterpipe smoking induces epigenetic changes in the small airway epithelium', PLoS One, vol. 12, no. 3, e0171112. https://doi.org/10.1371/journal.pone.0171112
Walters MS, Salit J, Ju JH, Staudt MR, Kaner RJ, Rogalski AM et al. Waterpipe smoking induces epigenetic changes in the small airway epithelium. PLoS One. 2017 Mar 1;12(3). e0171112. https://doi.org/10.1371/journal.pone.0171112
Walters, Matthew S. ; Salit, Jacqueline ; Ju, Jin Hyun ; Staudt, Michelle R. ; Kaner, Robert J. ; Rogalski, Allison M. ; Sodeinde, Teniola B. ; Rahim, Riyaad ; Strulovici-Barel, Yael ; Mezey, Jason G. ; Almulla, Ahmad M. ; Sattar, Hisham ; Mahmoud, Mai ; Crystal, Ronald. / Waterpipe smoking induces epigenetic changes in the small airway epithelium. In: PLoS One. 2017 ; Vol. 12, No. 3.
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