Vpr and Vpu are important for efficient human immunodeficiency virus type 1 replication and CD4+ T-cell depletion in human lymphoid tissue ex vivo

Elke Rücker, Jean Charles Grivel, Jan Münch, Frank Kirchhoff, Leonid Margolis

Research output: Contribution to journalArticle

30 Citations (Scopus)


The relevance of the accessory vpr, vpu, and nef genes for human immunodeficiency virus type 1 (HIV-1) replication in human lymphoid tissue (HLT), the major site of viral replication in vivo, is largely unknown. Here, we show that an individual deletion of nef, vpr, or vpu significantly decreases HIV-1 replication and prevents CD4+ T-cell depletion in ex vivo HLT. However, only combined defects in all three accessory genes entirely disrupt the replicative capacity of HIV-1. Our results demonstrate that nef, vpr, and vpu are all essential for efficient viral spread in HLT, suggesting an important role in AIDS pathogenesis.

Original languageEnglish
Pages (from-to)12689-12693
Number of pages5
JournalJournal of Virology
Issue number22
Publication statusPublished - 1 Nov 2004


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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