Vpr and Vpu are important for efficient human immunodeficiency virus type 1 replication and CD4+ T-cell depletion in human lymphoid tissue ex vivo

Elke Rücker, Jean-Charles B. Grivel, Jan Münch, Frank Kirchhoff, Leonid Margolis

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The relevance of the accessory vpr, vpu, and nef genes for human immunodeficiency virus type 1 (HIV-1) replication in human lymphoid tissue (HLT), the major site of viral replication in vivo, is largely unknown. Here, we show that an individual deletion of nef, vpr, or vpu significantly decreases HIV-1 replication and prevents CD4+ T-cell depletion in ex vivo HLT. However, only combined defects in all three accessory genes entirely disrupt the replicative capacity of HIV-1. Our results demonstrate that nef, vpr, and vpu are all essential for efficient viral spread in HLT, suggesting an important role in AIDS pathogenesis.

Original languageEnglish
Pages (from-to)12689-12693
Number of pages5
JournalJournal of Virology
Volume78
Issue number22
DOIs
Publication statusPublished - Nov 2004
Externally publishedYes

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Lymphoid Tissue
Virus Replication
HIV-1
T-Lymphocytes
vpu Genes
nef Genes
vpr Genes
Acquired Immunodeficiency Syndrome
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Vpr and Vpu are important for efficient human immunodeficiency virus type 1 replication and CD4+ T-cell depletion in human lymphoid tissue ex vivo. / Rücker, Elke; Grivel, Jean-Charles B.; Münch, Jan; Kirchhoff, Frank; Margolis, Leonid.

In: Journal of Virology, Vol. 78, No. 22, 11.2004, p. 12689-12693.

Research output: Contribution to journalArticle

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