Vitamin C enhances the expression of IL17 in a Jmjd2-dependent manner

Mi Hye Song, Varun Sasidharan Nair, Kwon Ik Oh

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Previously, we reported that vitamin C facilitates the CpG demethylation of Foxp3 enhancer in CD4+Foxp3+ regulatory T cells (Tregs) by enhancing the activity of a DNA demethylase ten-eleven-translocation (Tet). However, it is not clear whether vitamin C affects other helper T cell lineages like T helper type 17 (Th17) cells which are related with Tregs. Here, we show that the expression of interleukin-17A (IL17) increases with the treatment of vitamin C but not with other antioxidants. Interestingly, the upregulation of IL17 was not accompanied by DNA demethylation in Il17 promoter and was independent of Tet enzymes. Rather, vitamin C reduced the trimethylation of histone H3 lysine 9 (H3K9me3) in the regulatory elements of the Il17 locus, and the effects of vitamin C were abrogated by knockdown of jumonji-C domain-containing protein 2 (jmjd2). These results suggest that vitamin C can affect the expression of IL17 by modulating the histone demethylase activity.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalBMB Reports
Volume50
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

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Keywords

  • H3K9me3
  • Histone modification
  • IL17
  • Jmjd2
  • Vitamin C

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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