Variations in gene expression among different types of human skeletal muscle

Peter B. Kang, Alvin T. Kho, Despina Sanoudou, Judith N. Haslett, Chad P. Dow, Mei Han, Jessica M. Blasko, Hart G.W. Lidov, Alan H. Beggs, Louis M. Kunkel

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    There is a consistent variation in the response of different skeletal muscle groups to mutations in genes known to cause muscular dystrophy, yet these muscles appear histologically similar. To better understand these phenotypic differences, we analyzed gene expression patterns in control muscle specimens obtained from four sites at autopsy: deltoid, quadriceps, gastrocnemius, and tibialis anterior (TA). A total of 35 muscle samples from nine individuals (four pediatric and five geriatric) were studied. Factors potentially influencing gene expression in the different samples included individuality, age, muscle type, gender, cause of death, postmortem interval, and ethnicity. The first three factors, in decreasing order, were found to have a significant impact on the stratification of muscle specimens. A novel analytic method, using a second round of normalization, was used to elicit differences between muscle types. This approach may be extended to a broader survey, potentially elucidating a molecular classification of the skeletal muscles.

    Original languageEnglish
    Pages (from-to)483-491
    Number of pages9
    JournalMuscle and Nerve
    Volume32
    Issue number4
    DOIs
    Publication statusPublished - 1 Oct 2005

    Keywords

    • Aging
    • Gene expression
    • Muscle
    • Muscular dystrophy

    ASJC Scopus subject areas

    • Physiology
    • Clinical Neurology
    • Cellular and Molecular Neuroscience
    • Physiology (medical)

    Fingerprint Dive into the research topics of 'Variations in gene expression among different types of human skeletal muscle'. Together they form a unique fingerprint.

  • Cite this

    Kang, P. B., Kho, A. T., Sanoudou, D., Haslett, J. N., Dow, C. P., Han, M., Blasko, J. M., Lidov, H. G. W., Beggs, A. H., & Kunkel, L. M. (2005). Variations in gene expression among different types of human skeletal muscle. Muscle and Nerve, 32(4), 483-491. https://doi.org/10.1002/mus.20356