Validation of electrochemiluminescence assays for highly sensitive and reproducible quantification of a-synuclein in cerebrospinal fluid

Niels Kruse, Omar Ali El-Agnaf, Brit Mollenhauer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

α-Synuclein (aSyn), a putative cerebrospinal fluid biomarker, may support the diagnosis of neurodegenerative diseases. Previous studies led to conflicting results due to different preanalytical and analytical procedures. Standardized assays are required to allow for comparison of results from different laboratories. Materials & methods: We performed a side-by-side validation of a commercially available (Meso Scale Discovery, MD, USA) and a 'homebrew' assay for quantification of aSyn according to published guidelines. Results: The data showed high sensitivity and reproducibility for both assays. Preanalytical and analytical parameters did not affect the outcome of measurements. Conclusion: We conclude that both assays are very close in performance and suitable for research application of cerebrospinal fluid aSyn.

Original languageEnglish
Pages (from-to)621-630
Number of pages10
JournalBioanalysis
Volume9
Issue number8
DOIs
Publication statusPublished - 1 Apr 2017
Externally publishedYes

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Keywords

  • Assay
  • Electrochemiluminescence
  • Validation
  • α-synuclein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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