Vacuolar-type H+-ATPase distribution in unstimulated and acetylcholine-activated isolated human eccrine sweat glands

Douglas Bovell, M. T. Clunes, E. Roussa, J. Burry, H. Y. Elder

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The presence and cellular distribution of subunits of the V1 sector of the vacuolar-type H+-ATPase (V-ATPase) was investigated in isolated human eccrine sweat glands. In every instance, V-ATPase was located in the cytoplasm and apical membranes of the luminal cells of the reabsorptive duct segment. In the secretory coil, both diffuse and perinuclear staining was demonstrated in the secretory cells, with additional expression at the apical and basolateral membranes and on the inter-cellular canaliculi. There was no detectable difference in V-ATPase expression as a result of prior application of 100 μM acetylcholine.

Original languageEnglish
Pages (from-to)409-413
Number of pages5
JournalHistochemical Journal
Volume32
Issue number7
DOIs
Publication statusPublished - 2000
Externally publishedYes

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Eccrine Glands
Vacuolar Proton-Translocating ATPases
Sweat Glands
Acetylcholine
Cytoplasm
Cell Membrane
Staining and Labeling
Membranes

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Vacuolar-type H+-ATPase distribution in unstimulated and acetylcholine-activated isolated human eccrine sweat glands. / Bovell, Douglas; Clunes, M. T.; Roussa, E.; Burry, J.; Elder, H. Y.

In: Histochemical Journal, Vol. 32, No. 7, 2000, p. 409-413.

Research output: Contribution to journalArticle

Bovell, Douglas ; Clunes, M. T. ; Roussa, E. ; Burry, J. ; Elder, H. Y. / Vacuolar-type H+-ATPase distribution in unstimulated and acetylcholine-activated isolated human eccrine sweat glands. In: Histochemical Journal. 2000 ; Vol. 32, No. 7. pp. 409-413.
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