Vaccenic acid suppresses intestinal inflammation by increasing anandamide and related N-acylethanolamines in the JCR: LA-cp rat

Miriam Jacome-Sosa, Claudia Vacca, Rabban Mangat, Abdoulaye Diane, Randy C. Nelson, Martin J. Reaney, Jianheng Shen, Jonathan M. Curtis, Donna F. Vine, Catherine J. Field, Miki Igarashi, Daniele Piomelli, Sebastiano Banni, Spencer D. Proctor

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Abstract

Vaccenic acid (VA), the predominant ruminantderived trans fat in the food chain, ameliorates hyperlipidemia, yet mechanisms remain elusive. We investigated whether VA could influence tissue endocannabinoids (ECs) by altering the availability of their biosynthetic precursor, arachidonic acid (AA), in membrane phospholipids (PLs). JCR:LA-cp rats were assigned to a control diet with or without VA (1% w/w), cis-9, trans-11 conjugated linoleic acid (CLA) (1% w/w) or VA+CLA (1% + 0.5% w/w) for 8 weeks. VA reduced the EC, 2-arachidonoylglycerol (2-AG), in the liver and visceral adipose tissue (VAT) relative to control diet (P < 0.001), but did not change AA in tissue PLs. There was no additive effect of combining VA+CLA on 2-AG relative to VA alone (P > 0.05). Interestingly, VA increased jejunal concentrations of anandamide and those of the noncannabinoid signaling molecules, oleoylethanolamide and palmitoylethanolamide, relative to control diet (P < 0.05). This was consistent with a lower jejunal protein abundance (but not activity) of their degrading enzyme, fatty acid amide hydrolase, as well as the mRNA expression of TNFα and interleukin 1β (P < 0.05). The ability of VA to reduce 2-AG in the liver and VAT provides a potential mechanistic explanation to alleviate ectopic lipid accumulation. The opposing regulation of ECs and other noncannabinoid lipid signaling molecules by VA suggests an activation of benefit via the EC system in the intestine.

Original languageEnglish
Pages (from-to)638-649
Number of pages12
JournalJournal of Lipid Research
Volume57
Issue number4
DOIs
Publication statusPublished - 1 Apr 2016
Externally publishedYes

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Keywords

  • Anandamide
  • Endocannabinoids
  • Intestinal inflammatory diseases
  • N-acylethanolamines
  • Vaccenic acid

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Jacome-Sosa, M., Vacca, C., Mangat, R., Diane, A., Nelson, R. C., Reaney, M. J., Shen, J., Curtis, J. M., Vine, D. F., Field, C. J., Igarashi, M., Piomelli, D., Banni, S., & Proctor, S. D. (2016). Vaccenic acid suppresses intestinal inflammation by increasing anandamide and related N-acylethanolamines in the JCR: LA-cp rat. Journal of Lipid Research, 57(4), 638-649. https://doi.org/10.1194/jlr.M066308