UV and visible light screening by individual sporopollenin exines derived from Lycopodium clavatum (club moss) and Ambrosia trifida (giant ragweed)

Stephen Atkin, Sylvain Barrier, Zhenggang Cui, Paul D I Fletcher, Grahame MacKenzie, Vincent Panel, Vincent Sol, Xunli Zhang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have investigated the UV-visible light transmission of three types of micrometre-sized sporopollenin exine shells, two derived from Lycopodium clavatum (club moss) spores and one from Ambrosia trifida (giant ragweed) pollen. We have used spectrophotometer measurements of partial monolayers of exines and microscope absorbance imaging to derive the light transmission properties of individual exines. Measurements have been made for exines in air when light transmission losses are due to a combination of absorption, reflection and scattering processes and for exines dispersed in a liquid for which the refractive index (RI) is approximately equal to the RI of the exine such that reflection and scattering effects are negligible. Overall, it found that the light transmission of a single exine wall is approximately 50%. This value of the transmission is due mainly to light absorption, is similar for the three exines studied here and varies only slightly with light wavelength over the range 200-900 nm.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalJournal of Photochemistry and Photobiology B: Biology
Volume102
Issue number3
DOIs
Publication statusPublished - 2 Mar 2011
Externally publishedYes

Fingerprint

Lycopodiaceae
Lycopodium
Ambrosia
Bryophytes
light transmission
Ultraviolet Rays
screening
Light
Refractometry
refractivity
pollen
spores
transmission loss
spectrophotometers
electromagnetic absorption
scattering
micrometers
Spores
microscopes
Air

Keywords

  • Plant spores
  • Pollen
  • Screening
  • Sporopollenin
  • UV-visible light absorbance
  • UV-visible light transmission

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

UV and visible light screening by individual sporopollenin exines derived from Lycopodium clavatum (club moss) and Ambrosia trifida (giant ragweed). / Atkin, Stephen; Barrier, Sylvain; Cui, Zhenggang; Fletcher, Paul D I; MacKenzie, Grahame; Panel, Vincent; Sol, Vincent; Zhang, Xunli.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 102, No. 3, 02.03.2011, p. 209-217.

Research output: Contribution to journalArticle

Atkin, Stephen ; Barrier, Sylvain ; Cui, Zhenggang ; Fletcher, Paul D I ; MacKenzie, Grahame ; Panel, Vincent ; Sol, Vincent ; Zhang, Xunli. / UV and visible light screening by individual sporopollenin exines derived from Lycopodium clavatum (club moss) and Ambrosia trifida (giant ragweed). In: Journal of Photochemistry and Photobiology B: Biology. 2011 ; Vol. 102, No. 3. pp. 209-217.
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