Utilization of Pistia stratiotes (aquatic weed) for fermentative biohydrogen

Electron-equivalent balance, stoichiometry, and cost estimation

Nonsikelelo Precios Mthethwa, Mahmoud Nasr, Faizal Bux, Sheena Kumari

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study investigated the utilization of Pistia stratiotes for biohydrogen production via a dark-fermentation process. The aquatic plant was subjected to acid-hydrolysis using H2SO4: 3.0% (v/v) for 40 min, resulting in sugar yield: 122.2 ± 5.2 mg/g. The optimum culture pH was 5.5, achieving hydrogen yield (HY): 2.46 ± 0.14 mol-H2/mol-glucose (3.51 ± 0.20 mg-H2/g-dry weight) at fermentation time 8 h, temperature 25 °C, and substrate-to-biomass (S/X) ratio 1.0 g-COD/g-VSS. The organic mass balance (92–96%) and electron-equivalent balance (92–98%) indicated the reliability of fermentation data. The dominant species included Planctomycetales, Verrucomicrobiales, Clostridiaceae, and Gammaproteobacteria. The phylogenetic analysis confirmed the abundance of hydrogen-producing bacteria such as Bacillus, Clostridium, and Enterobacter. The hydrogenase gene expression provided the highest activity at pH: 5.5 with a cell number 2.53 × 104 copies/ng-DNA compared to pH: 4.5 (6.95 × 103 copies/ng-DNA) and pH: 8.5 (7.77 × 103 copies/ng-DNA). The total cost of the fermentation system including the amortization cost of investment and operating cost was 0.08 $/kg-dry weight (22.8 $/kg-H2 produced).

Original languageEnglish
Pages (from-to)8243-8255
Number of pages13
JournalInternational Journal of Hydrogen Energy
Volume43
Issue number17
DOIs
Publication statusPublished - 26 Apr 2018

Fingerprint

fermentation
Stoichiometry
Fermentation
stoichiometry
costs
DNA
deoxyribonucleic acid
Electrons
Costs
electrons
aquatic plants
Clostridium
operating costs
Hydrogen
crack opening displacement
Bacillus
gene expression
mass balance
Bacilli
hydrogen

Keywords

  • Bacterial community
  • Biohydrogen cost
  • Dark-fermentation
  • Full stoichiometry
  • Pistia stratiotes substrate

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Utilization of Pistia stratiotes (aquatic weed) for fermentative biohydrogen : Electron-equivalent balance, stoichiometry, and cost estimation. / Mthethwa, Nonsikelelo Precios; Nasr, Mahmoud; Bux, Faizal; Kumari, Sheena.

In: International Journal of Hydrogen Energy, Vol. 43, No. 17, 26.04.2018, p. 8243-8255.

Research output: Contribution to journalArticle

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abstract = "This study investigated the utilization of Pistia stratiotes for biohydrogen production via a dark-fermentation process. The aquatic plant was subjected to acid-hydrolysis using H2SO4: 3.0{\%} (v/v) for 40 min, resulting in sugar yield: 122.2 ± 5.2 mg/g. The optimum culture pH was 5.5, achieving hydrogen yield (HY): 2.46 ± 0.14 mol-H2/mol-glucose (3.51 ± 0.20 mg-H2/g-dry weight) at fermentation time 8 h, temperature 25 °C, and substrate-to-biomass (S/X) ratio 1.0 g-COD/g-VSS. The organic mass balance (92–96{\%}) and electron-equivalent balance (92–98{\%}) indicated the reliability of fermentation data. The dominant species included Planctomycetales, Verrucomicrobiales, Clostridiaceae, and Gammaproteobacteria. The phylogenetic analysis confirmed the abundance of hydrogen-producing bacteria such as Bacillus, Clostridium, and Enterobacter. The hydrogenase gene expression provided the highest activity at pH: 5.5 with a cell number 2.53 × 104 copies/ng-DNA compared to pH: 4.5 (6.95 × 103 copies/ng-DNA) and pH: 8.5 (7.77 × 103 copies/ng-DNA). The total cost of the fermentation system including the amortization cost of investment and operating cost was 0.08 $/kg-dry weight (22.8 $/kg-H2 produced).",
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