Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística.

Translated title of the contribution: Use of the polymerase chain reaction technic in the genetic analysis of cystic fibrosis

M. Chillón, T. Casals, F. J. Giménez, H. Badía, N. Morral, A. Bosch, V. Nunes, Xavier P. Estivill

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The development of polymerase chain reaction (PCR) which allows the specific amplification of DNA sequences has improved considerably the genetic analysis of hereditary diseases. We present here the application of this new technique to the genetic analysis of cystic fibrosis (CF), the most frequent severe genetic disease in caucasians. We have amplified four sequences containing polymorphisms linked to the CF gene (CS.7, KM.19, MP6d-9 and J3.11), and analysed the amplified products with restriction enzymes. Complete concordance was found with classical Southern methods, allowing the application of PCR to routine CF family studies.

Original languageSpanish
Pages (from-to)444-447
Number of pages4
JournalMedicina Clinica
Volume94
Issue number12
Publication statusPublished - 31 Mar 1990
Externally publishedYes

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Genetic Techniques
Cystic Fibrosis
Inborn Genetic Diseases
Polymerase Chain Reaction
Enzymes
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chillón, M., Casals, T., Giménez, F. J., Badía, H., Morral, N., Bosch, A., ... Estivill, X. P. (1990). Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística. Medicina Clinica, 94(12), 444-447.

Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística. / Chillón, M.; Casals, T.; Giménez, F. J.; Badía, H.; Morral, N.; Bosch, A.; Nunes, V.; Estivill, Xavier P.

In: Medicina Clinica, Vol. 94, No. 12, 31.03.1990, p. 444-447.

Research output: Contribution to journalArticle

Chillón, M, Casals, T, Giménez, FJ, Badía, H, Morral, N, Bosch, A, Nunes, V & Estivill, XP 1990, 'Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística.', Medicina Clinica, vol. 94, no. 12, pp. 444-447.
Chillón M, Casals T, Giménez FJ, Badía H, Morral N, Bosch A et al. Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística. Medicina Clinica. 1990 Mar 31;94(12):444-447.
Chillón, M. ; Casals, T. ; Giménez, F. J. ; Badía, H. ; Morral, N. ; Bosch, A. ; Nunes, V. ; Estivill, Xavier P. / Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística. In: Medicina Clinica. 1990 ; Vol. 94, No. 12. pp. 444-447.
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