Abstract
The development of polymerase chain reaction (PCR) which allows the specific amplification of DNA sequences has improved considerably the genetic analysis of hereditary diseases. We present here the application of this new technique to the genetic analysis of cystic fibrosis (CF), the most frequent severe genetic disease in caucasians. We have amplified four sequences containing polymorphisms linked to the CF gene (CS.7, KM.19, MP6d-9 and J3.11), and analysed the amplified products with restriction enzymes. Complete concordance was found with classical Southern methods, allowing the application of PCR to routine CF family studies.
| Original language | Spanish |
|---|---|
| Pages (from-to) | 444-447 |
| Number of pages | 4 |
| Journal | Medicina Clinica |
| Volume | 94 |
| Issue number | 12 |
| Publication status | Published - 31 Mar 1990 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Medicine(all)
Cite this
Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística. / Chillón, M.; Casals, T.; Giménez, F. J.; Badía, H.; Morral, N.; Bosch, A.; Nunes, V.; Estivill, Xavier P.
In: Medicina Clinica, Vol. 94, No. 12, 31.03.1990, p. 444-447.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística.
AU - Chillón, M.
AU - Casals, T.
AU - Giménez, F. J.
AU - Badía, H.
AU - Morral, N.
AU - Bosch, A.
AU - Nunes, V.
AU - Estivill, Xavier P.
PY - 1990/3/31
Y1 - 1990/3/31
N2 - The development of polymerase chain reaction (PCR) which allows the specific amplification of DNA sequences has improved considerably the genetic analysis of hereditary diseases. We present here the application of this new technique to the genetic analysis of cystic fibrosis (CF), the most frequent severe genetic disease in caucasians. We have amplified four sequences containing polymorphisms linked to the CF gene (CS.7, KM.19, MP6d-9 and J3.11), and analysed the amplified products with restriction enzymes. Complete concordance was found with classical Southern methods, allowing the application of PCR to routine CF family studies.
AB - The development of polymerase chain reaction (PCR) which allows the specific amplification of DNA sequences has improved considerably the genetic analysis of hereditary diseases. We present here the application of this new technique to the genetic analysis of cystic fibrosis (CF), the most frequent severe genetic disease in caucasians. We have amplified four sequences containing polymorphisms linked to the CF gene (CS.7, KM.19, MP6d-9 and J3.11), and analysed the amplified products with restriction enzymes. Complete concordance was found with classical Southern methods, allowing the application of PCR to routine CF family studies.
UR - http://www.scopus.com/inward/record.url?scp=0025717110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025717110&partnerID=8YFLogxK
M3 - Article
VL - 94
SP - 444
EP - 447
JO - Medicina Clinica
JF - Medicina Clinica
SN - 0025-7753
IS - 12
ER -