Urine biomarkers informative of human kidney allograft rejection and tolerance

Voravech Nissaisorakarn, John Richard Lee, Michelle Lubetzky, Manikkam Suthanthiran

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed urinary cell messenger RNA (mRNA) profiling to monitor in vivo status of human kidney allografts based on our conceptualization that the kidney allograft may function as an in vivo flow cell sorter allowing access of graft infiltrating cells to the glomerular ultrafiltrate and that interrogation of urinary cells is informative of allograft status. For the profiling urinary cells, we developed a two-step preamplification enhanced real-time quantitative PCR (RT-QPCR) assays with a customized amplicon; preamplification compensating for the low RNA yield from urine and the customized amplicon facilitating absolute quantification of mRNA and overcoming the inherent limitations of relative quantification widely used in RT-QPCR assays. Herein, we review our discovery and validation of urinary cell mRNAs as noninvasive biomarkers prognostic and diagnostic of acute cellular rejection (ACR) in kidney allografts. We summarize our results reflecting the utility of urinary cell mRNA profiling for predicting reversal of ACR with anti-rejection therapy; differential diagnosis of kidney allograft dysfunction; and noninvasive diagnosis and prognosis of BK virus nephropathy. Messenger RNA profiles associated with human kidney allograft tolerance are also summarized in this review. Altogether, data supporting the idea that urinary cell mRNA profiles are informative of kidney allograft status and tolerance are reviewed in this report.

Original languageEnglish
JournalHuman Immunology
DOIs
Publication statusAccepted/In press - 1 Jan 2018
Externally publishedYes

Fingerprint

Transplantation Tolerance
Biomarkers
Urine
Kidney
Allografts
Messenger RNA
Real-Time Polymerase Chain Reaction
BK Virus
Immunosuppression
Differential Diagnosis
RNA
Transplants

Keywords

  • Kidney transplantation
  • Polymerase chain reaction
  • Rejection
  • Tolerance
  • Urinary cell mRNA profiling

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Urine biomarkers informative of human kidney allograft rejection and tolerance. / Nissaisorakarn, Voravech; Lee, John Richard; Lubetzky, Michelle; Suthanthiran, Manikkam.

In: Human Immunology, 01.01.2018.

Research output: Contribution to journalArticle

Nissaisorakarn, Voravech ; Lee, John Richard ; Lubetzky, Michelle ; Suthanthiran, Manikkam. / Urine biomarkers informative of human kidney allograft rejection and tolerance. In: Human Immunology. 2018.
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