Uptake and release of Ca2+ by the endoplasmic reticulum contribute to the oscillations of the cytosolic Ca2+ concentration triggered by Ca2+ influx in the electrically excitable pancreatic B-cell

Patrick Gilon, Abdelilah Arredouani, Philippe Gailly, Jesper Gromada, Jean Claude Henquin

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The role of intracellular Ca2+ pools in oscillations of the cytosolic Ca2+ concentration ([Ca2+](c)) triggered by Ca2+ influx was investigated in mouse pancreatic B-cells. [Ca2+](c) oscillations occurring spontaneously during glucose stimulation or repetitively induced by pulses of high K+ (in the presence of diazoxide) were characterized by a descending phase in two components. A rapid decrease in [Ca2+](c) coincided with closure of voltage-dependent Ca2+ channels and was followed by a slower phase independent of Ca2+ influx. Blocking the SERCA pump with thapsigargin or cyclopiazonic acid accelerated the rising phase of [Ca2+](c) oscillations and increased their amplitude, which suggests that the endoplasmic reticulum (ER) rapidly takes up Ca2+. It also suppressed the slow [Ca2+](c) recovery phase, which indicates that this phase corresponds to the slow release of Ca2+ that was taken up by the ER during the upstroke of the [Ca2+](c) transient. Glucose promoted the buffering capacity of the ER and amplified the slow [Ca2+](c) recovery phase. The slow phase induced by high K+ pulses was not affected by modulators of Ca2+- or inositol 1,4,5-trisphosphate-induced Ca2+ release, did not involve a depolarization- induced Ca2+ release, and was also observed at the end of a rapid rise in [Ca2+](c) triggered from caged Ca2+. It is attributed to passive leakage of Ca2+ from the ER. We suggest that the ER displays oscillations of the Ca2+ concentration ([Ca2+](ER)) concomitant and parallel to [Ca2+](c). The observation that thapsigargin depolarizes the membrane of B-cells supports the proposal that the degree of Ca2+ filling of the ER modulates the membrane potential. Therefore, [Ca2+](ER) oscillations occurring during glucose stimulation are likely to influence the bursting behavior of B-cells and eventually [Ca2+](c) oscillations.

Original languageEnglish
Pages (from-to)20197-20205
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number29
DOIs
Publication statusPublished - 16 Jul 1999
Externally publishedYes

Fingerprint

Insulin-Secreting Cells
Endoplasmic Reticulum
Thapsigargin
Cells
Glucose
Membranes
Diazoxide
Recovery
Inositol 1,4,5-Trisphosphate
Depolarization
Modulators
Pumps
B-Lymphocytes
Electric potential
Membrane Potentials

ASJC Scopus subject areas

  • Biochemistry

Cite this

Uptake and release of Ca2+ by the endoplasmic reticulum contribute to the oscillations of the cytosolic Ca2+ concentration triggered by Ca2+ influx in the electrically excitable pancreatic B-cell. / Gilon, Patrick; Arredouani, Abdelilah; Gailly, Philippe; Gromada, Jesper; Henquin, Jean Claude.

In: Journal of Biological Chemistry, Vol. 274, No. 29, 16.07.1999, p. 20197-20205.

Research output: Contribution to journalArticle

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abstract = "The role of intracellular Ca2+ pools in oscillations of the cytosolic Ca2+ concentration ([Ca2+](c)) triggered by Ca2+ influx was investigated in mouse pancreatic B-cells. [Ca2+](c) oscillations occurring spontaneously during glucose stimulation or repetitively induced by pulses of high K+ (in the presence of diazoxide) were characterized by a descending phase in two components. A rapid decrease in [Ca2+](c) coincided with closure of voltage-dependent Ca2+ channels and was followed by a slower phase independent of Ca2+ influx. Blocking the SERCA pump with thapsigargin or cyclopiazonic acid accelerated the rising phase of [Ca2+](c) oscillations and increased their amplitude, which suggests that the endoplasmic reticulum (ER) rapidly takes up Ca2+. It also suppressed the slow [Ca2+](c) recovery phase, which indicates that this phase corresponds to the slow release of Ca2+ that was taken up by the ER during the upstroke of the [Ca2+](c) transient. Glucose promoted the buffering capacity of the ER and amplified the slow [Ca2+](c) recovery phase. The slow phase induced by high K+ pulses was not affected by modulators of Ca2+- or inositol 1,4,5-trisphosphate-induced Ca2+ release, did not involve a depolarization- induced Ca2+ release, and was also observed at the end of a rapid rise in [Ca2+](c) triggered from caged Ca2+. It is attributed to passive leakage of Ca2+ from the ER. We suggest that the ER displays oscillations of the Ca2+ concentration ([Ca2+](ER)) concomitant and parallel to [Ca2+](c). The observation that thapsigargin depolarizes the membrane of B-cells supports the proposal that the degree of Ca2+ filling of the ER modulates the membrane potential. Therefore, [Ca2+](ER) oscillations occurring during glucose stimulation are likely to influence the bursting behavior of B-cells and eventually [Ca2+](c) oscillations.",
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