Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo

Angélique Biancotto, Sarah J. Iglehart, Andrea Lisco, Christophe Vanpouille, Jean-Charles B. Grivel, Nell S. Lurain, Patricia S. Reichelderfer, Leonid B. Margolis

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

HIV-1 copathogens are believed to play a critical role in progression to AIDS. Human cytomegalovirus (HCMV) has a high prevalence in the general population and is a common copathogen in HIV-1-infected individuals. Important events in copathogen interactions with HIV-1 take place in lymphoid tissue where critical events in HIV-1 disease occur. Here, we used an experimental system of human lymphoid tissue ex vivo to investigate interactions of HCMV with HIV-1. We inoculated ex vivoblocks of human lymphoid tissue with a recombinant strain of HCMV, expressing the green fluorescent protein, and HIV-1 and monitored viral replication and the phenotype of productively infected cells. HCMV readily replicated in tissue blocks as revealed by the release of HCMV viral DNA and an increasing number of viral-positive cells. Immunophenotyping of HCMV-infected cells showed a preferential infection of activated lymphocytes. The number of these cells significantly increased in HIV-1-coinfected tissues. Accordingly, HCMV replication was enhanced 2- to-3 fold. This upregulation occurred in tissues infected with either CXCR4- or CCR5-utilizing HIV-1. Thus, HIV-1 creates new targets for HCMV, which may explain the strong association of HCMV with HIV-1 infection in vivo. Ex vivo-infected human lymphoid tissue constitutes a model to study the mechanisms of HCMV tissue pathogenesis and its interactions with HIV-1 and this model may provide new targets for anti-HIV-1 therapy.

Original languageEnglish
Pages (from-to)453-462
Number of pages10
JournalAIDS Research and Human Retroviruses
Volume24
Issue number3
DOIs
Publication statusPublished - 1 Mar 2008
Externally publishedYes

Fingerprint

Lymphoid Tissue
Cytomegalovirus
HIV-1
Up-Regulation
Immunophenotyping
Viral DNA
Green Fluorescent Proteins
HIV Infections
Acquired Immunodeficiency Syndrome
Cell Count
Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Infectious Diseases
  • Virology

Cite this

Biancotto, A., Iglehart, S. J., Lisco, A., Vanpouille, C., Grivel, J-C. B., Lurain, N. S., ... Margolis, L. B. (2008). Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Research and Human Retroviruses, 24(3), 453-462. https://doi.org/10.1089/aid.2007.0155

Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. / Biancotto, Angélique; Iglehart, Sarah J.; Lisco, Andrea; Vanpouille, Christophe; Grivel, Jean-Charles B.; Lurain, Nell S.; Reichelderfer, Patricia S.; Margolis, Leonid B.

In: AIDS Research and Human Retroviruses, Vol. 24, No. 3, 01.03.2008, p. 453-462.

Research output: Contribution to journalArticle

Biancotto, A, Iglehart, SJ, Lisco, A, Vanpouille, C, Grivel, J-CB, Lurain, NS, Reichelderfer, PS & Margolis, LB 2008, 'Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo', AIDS Research and Human Retroviruses, vol. 24, no. 3, pp. 453-462. https://doi.org/10.1089/aid.2007.0155
Biancotto, Angélique ; Iglehart, Sarah J. ; Lisco, Andrea ; Vanpouille, Christophe ; Grivel, Jean-Charles B. ; Lurain, Nell S. ; Reichelderfer, Patricia S. ; Margolis, Leonid B. / Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. In: AIDS Research and Human Retroviruses. 2008 ; Vol. 24, No. 3. pp. 453-462.
@article{85461cbda5f447d48d1019f10ed3d616,
title = "Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo",
abstract = "HIV-1 copathogens are believed to play a critical role in progression to AIDS. Human cytomegalovirus (HCMV) has a high prevalence in the general population and is a common copathogen in HIV-1-infected individuals. Important events in copathogen interactions with HIV-1 take place in lymphoid tissue where critical events in HIV-1 disease occur. Here, we used an experimental system of human lymphoid tissue ex vivo to investigate interactions of HCMV with HIV-1. We inoculated ex vivoblocks of human lymphoid tissue with a recombinant strain of HCMV, expressing the green fluorescent protein, and HIV-1 and monitored viral replication and the phenotype of productively infected cells. HCMV readily replicated in tissue blocks as revealed by the release of HCMV viral DNA and an increasing number of viral-positive cells. Immunophenotyping of HCMV-infected cells showed a preferential infection of activated lymphocytes. The number of these cells significantly increased in HIV-1-coinfected tissues. Accordingly, HCMV replication was enhanced 2- to-3 fold. This upregulation occurred in tissues infected with either CXCR4- or CCR5-utilizing HIV-1. Thus, HIV-1 creates new targets for HCMV, which may explain the strong association of HCMV with HIV-1 infection in vivo. Ex vivo-infected human lymphoid tissue constitutes a model to study the mechanisms of HCMV tissue pathogenesis and its interactions with HIV-1 and this model may provide new targets for anti-HIV-1 therapy.",
author = "Ang{\'e}lique Biancotto and Iglehart, {Sarah J.} and Andrea Lisco and Christophe Vanpouille and Grivel, {Jean-Charles B.} and Lurain, {Nell S.} and Reichelderfer, {Patricia S.} and Margolis, {Leonid B.}",
year = "2008",
month = "3",
day = "1",
doi = "10.1089/aid.2007.0155",
language = "English",
volume = "24",
pages = "453--462",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo

AU - Biancotto, Angélique

AU - Iglehart, Sarah J.

AU - Lisco, Andrea

AU - Vanpouille, Christophe

AU - Grivel, Jean-Charles B.

AU - Lurain, Nell S.

AU - Reichelderfer, Patricia S.

AU - Margolis, Leonid B.

PY - 2008/3/1

Y1 - 2008/3/1

N2 - HIV-1 copathogens are believed to play a critical role in progression to AIDS. Human cytomegalovirus (HCMV) has a high prevalence in the general population and is a common copathogen in HIV-1-infected individuals. Important events in copathogen interactions with HIV-1 take place in lymphoid tissue where critical events in HIV-1 disease occur. Here, we used an experimental system of human lymphoid tissue ex vivo to investigate interactions of HCMV with HIV-1. We inoculated ex vivoblocks of human lymphoid tissue with a recombinant strain of HCMV, expressing the green fluorescent protein, and HIV-1 and monitored viral replication and the phenotype of productively infected cells. HCMV readily replicated in tissue blocks as revealed by the release of HCMV viral DNA and an increasing number of viral-positive cells. Immunophenotyping of HCMV-infected cells showed a preferential infection of activated lymphocytes. The number of these cells significantly increased in HIV-1-coinfected tissues. Accordingly, HCMV replication was enhanced 2- to-3 fold. This upregulation occurred in tissues infected with either CXCR4- or CCR5-utilizing HIV-1. Thus, HIV-1 creates new targets for HCMV, which may explain the strong association of HCMV with HIV-1 infection in vivo. Ex vivo-infected human lymphoid tissue constitutes a model to study the mechanisms of HCMV tissue pathogenesis and its interactions with HIV-1 and this model may provide new targets for anti-HIV-1 therapy.

AB - HIV-1 copathogens are believed to play a critical role in progression to AIDS. Human cytomegalovirus (HCMV) has a high prevalence in the general population and is a common copathogen in HIV-1-infected individuals. Important events in copathogen interactions with HIV-1 take place in lymphoid tissue where critical events in HIV-1 disease occur. Here, we used an experimental system of human lymphoid tissue ex vivo to investigate interactions of HCMV with HIV-1. We inoculated ex vivoblocks of human lymphoid tissue with a recombinant strain of HCMV, expressing the green fluorescent protein, and HIV-1 and monitored viral replication and the phenotype of productively infected cells. HCMV readily replicated in tissue blocks as revealed by the release of HCMV viral DNA and an increasing number of viral-positive cells. Immunophenotyping of HCMV-infected cells showed a preferential infection of activated lymphocytes. The number of these cells significantly increased in HIV-1-coinfected tissues. Accordingly, HCMV replication was enhanced 2- to-3 fold. This upregulation occurred in tissues infected with either CXCR4- or CCR5-utilizing HIV-1. Thus, HIV-1 creates new targets for HCMV, which may explain the strong association of HCMV with HIV-1 infection in vivo. Ex vivo-infected human lymphoid tissue constitutes a model to study the mechanisms of HCMV tissue pathogenesis and its interactions with HIV-1 and this model may provide new targets for anti-HIV-1 therapy.

UR - http://www.scopus.com/inward/record.url?scp=41449088141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41449088141&partnerID=8YFLogxK

U2 - 10.1089/aid.2007.0155

DO - 10.1089/aid.2007.0155

M3 - Article

VL - 24

SP - 453

EP - 462

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 3

ER -