TRPM7 regulates polarized cell movements

Li Ting Su, Wei Liu, Hsiang Chin Chen, Omayra González-PAGÁN, Raymond Habas, Loren W. Runnels

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53 Citations (Scopus)


TRPM7 (transient receptor potential melastatin 7) is a Ca 2+-and Mg 2+-permeant ion channel in possession of its own kinase domain. As a kinase, the protein has been linked to the control of actomyosin contractility, whereas the channel has been found to regulate cell adhesion as well as cellular Mg 2+ homoeostasis. In the present study we show that depletion of TRPM7 by RNA interference in fibroblasts alters cell morphology, the cytoskeleton, and the ability of cells to form lamellipodia and to execute polarized cellmovements.Apulldown-purification assay revealed that knockdown of TRPM7 prevents cells from activating Rac and Cdc42 (cell division cycle 42) when stimulated to migrate into a cellular wound. Re-expression of TRPM7 reverses these phenotypic changes, as does, unexpectedly, expression of a kinaseinactive mutant of TRPM7. Surprisingly, expression of the Mg 2+ transporter SLC41A2 (solute carrier family 41 member 2) is also effective in restoring the change in cell morphology, disruption of the cytoskeleton and directional cell motility caused by depletion of the channel-kinase. The results of the present study uncover an essential role for Mg 2+ in the control of TRPM7 over the cytoskeleton and its ability to regulate polarized cell movements.

Original languageEnglish
Pages (from-to)513-521
Number of pages9
JournalBiochemical Journal
Issue number3
Publication statusPublished - 15 Mar 2011
Externally publishedYes



  • Cell morphology
  • Channel
  • Cytoskeleton
  • Magnesium
  • Polarized cell movement
  • Transient receptor potential melastatin 7 (TRPM7)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Su, L. T., Liu, W., Chen, H. C., González-PAGÁN, O., Habas, R., & Runnels, L. W. (2011). TRPM7 regulates polarized cell movements. Biochemical Journal, 434(3), 513-521.