Tropheryma whipplei Twist

A human pathogenic actinobacteria with a reduced genome

Didier Raoult, Hiroyuki Ogata, Stéphane Audic, Catherine Robert, Karsten Suhre, Michel Drancourt, Jean Michel Claverie

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

The human pathogen Tropheryma whipplei is the only known reduced genome species (<1 Mb) within the Actinobacteria [high G+C Gram-positive bacteria]. We present the sequence of the 927,303-bp circular genome of T. whipplei Twist strain, encoding 808 predicted protein-coding genes. Specific genome features include deficiencies in amino acid metabolisms, the lack of clear thioredoxin and thioredoxin reductase homologs, and a mutation in DNA gyrase predicting a resistance to quinolone antibiotics. Moreover, the alignment of the two available T. whipplei genome sequences (Twist vs. TW08/27) revealed a large chromosomal inversion the extremities of which are located within two paralogous genes. These genes belong to a large cell-surface protein family defined by the presence of a common repeat highly conserved at the nucleotide level. The repeats appear to trigger frequent genome rearrangements in T. whipplei, potentially resulting in the expression of different subsets of cell surface proteins. This might represent a new mechanism for evading host defenses. The T. whipplei genome sequence was also compared to other reduced bacterial genomes to examine the generality of previously detected features. The analysis of the genome sequence of this previously largely unknown human pathogen is now guiding the development of molecular diagnostic tools and more convenient culture conditions.

Original languageEnglish
Pages (from-to)1800-1809
Number of pages10
JournalGenome Research
Volume13
Issue number8
Publication statusPublished - 1 Aug 2003
Externally publishedYes

Fingerprint

Tropheryma
Actinobacteria
Genome
Membrane Proteins
DNA Gyrase
Thioredoxin-Disulfide Reductase
Bacterial Genomes
Thioredoxins
Molecular Pathology
Quinolones
Genes
Sequence Analysis
Extremities
Nucleotides
Anti-Bacterial Agents
Amino Acids
Mutation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Raoult, D., Ogata, H., Audic, S., Robert, C., Suhre, K., Drancourt, M., & Claverie, J. M. (2003). Tropheryma whipplei Twist: A human pathogenic actinobacteria with a reduced genome. Genome Research, 13(8), 1800-1809.

Tropheryma whipplei Twist : A human pathogenic actinobacteria with a reduced genome. / Raoult, Didier; Ogata, Hiroyuki; Audic, Stéphane; Robert, Catherine; Suhre, Karsten; Drancourt, Michel; Claverie, Jean Michel.

In: Genome Research, Vol. 13, No. 8, 01.08.2003, p. 1800-1809.

Research output: Contribution to journalArticle

Raoult, D, Ogata, H, Audic, S, Robert, C, Suhre, K, Drancourt, M & Claverie, JM 2003, 'Tropheryma whipplei Twist: A human pathogenic actinobacteria with a reduced genome', Genome Research, vol. 13, no. 8, pp. 1800-1809.
Raoult D, Ogata H, Audic S, Robert C, Suhre K, Drancourt M et al. Tropheryma whipplei Twist: A human pathogenic actinobacteria with a reduced genome. Genome Research. 2003 Aug 1;13(8):1800-1809.
Raoult, Didier ; Ogata, Hiroyuki ; Audic, Stéphane ; Robert, Catherine ; Suhre, Karsten ; Drancourt, Michel ; Claverie, Jean Michel. / Tropheryma whipplei Twist : A human pathogenic actinobacteria with a reduced genome. In: Genome Research. 2003 ; Vol. 13, No. 8. pp. 1800-1809.
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