Abstract
Tropheryma whipplei is a Gram positive human pathogen that is the causative agent of Whipple's disease. Nearly one century elapsed between the first description of the disease in 1907 and the cultivation of this bacterium within eukaryotic cell cultures in 2000. This achievement has made possible genome sequencing of this poorly studied microorganism. This review summarizes post-genomic knowledge resulting from these genomic data. To compare the theoretical genetic capabilities of T. whipplei with those of other sequenced bacteria, a virtual microarray representation was generated. This in silico analysis supports the concept of independent evolution pathways for microbial pathogens. Concrete post-genomic consequences related to clinical microbiology such as the analysis of antibiotic susceptibility or the design of molecular tools convenient for PCR detection and epidemiology studies are described. Analysis of whole-cell metabolic networks of T. whipplei also provide clues for designing axenic media for this pathogen that is particularly recalcitrant to cultivation. This opens the way to investigate transcriptome analysis of T. whipplei by microarrays. Future prospects are also discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 195-205 |
| Number of pages | 11 |
| Journal | Current Genomics |
| Volume | 6 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2005 |
| Externally published | Yes |
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Keywords
- Post-genomics
- Reduces genomes
- T. whipplei
ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
Cite this
Tropheryma whipplei genome at the beginning of the post-genomic era. / Crapoulet, N.; Renesto, P.; Dumler, J. S.; Suhre, Karsten; Ogata, H.; Claverie, J. M.; Raoult, Didier.
In: Current Genomics, Vol. 6, No. 3, 2005, p. 195-205.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Tropheryma whipplei genome at the beginning of the post-genomic era
AU - Crapoulet, N.
AU - Renesto, P.
AU - Dumler, J. S.
AU - Suhre, Karsten
AU - Ogata, H.
AU - Claverie, J. M.
AU - Raoult, Didier
PY - 2005
Y1 - 2005
N2 - Tropheryma whipplei is a Gram positive human pathogen that is the causative agent of Whipple's disease. Nearly one century elapsed between the first description of the disease in 1907 and the cultivation of this bacterium within eukaryotic cell cultures in 2000. This achievement has made possible genome sequencing of this poorly studied microorganism. This review summarizes post-genomic knowledge resulting from these genomic data. To compare the theoretical genetic capabilities of T. whipplei with those of other sequenced bacteria, a virtual microarray representation was generated. This in silico analysis supports the concept of independent evolution pathways for microbial pathogens. Concrete post-genomic consequences related to clinical microbiology such as the analysis of antibiotic susceptibility or the design of molecular tools convenient for PCR detection and epidemiology studies are described. Analysis of whole-cell metabolic networks of T. whipplei also provide clues for designing axenic media for this pathogen that is particularly recalcitrant to cultivation. This opens the way to investigate transcriptome analysis of T. whipplei by microarrays. Future prospects are also discussed.
AB - Tropheryma whipplei is a Gram positive human pathogen that is the causative agent of Whipple's disease. Nearly one century elapsed between the first description of the disease in 1907 and the cultivation of this bacterium within eukaryotic cell cultures in 2000. This achievement has made possible genome sequencing of this poorly studied microorganism. This review summarizes post-genomic knowledge resulting from these genomic data. To compare the theoretical genetic capabilities of T. whipplei with those of other sequenced bacteria, a virtual microarray representation was generated. This in silico analysis supports the concept of independent evolution pathways for microbial pathogens. Concrete post-genomic consequences related to clinical microbiology such as the analysis of antibiotic susceptibility or the design of molecular tools convenient for PCR detection and epidemiology studies are described. Analysis of whole-cell metabolic networks of T. whipplei also provide clues for designing axenic media for this pathogen that is particularly recalcitrant to cultivation. This opens the way to investigate transcriptome analysis of T. whipplei by microarrays. Future prospects are also discussed.
KW - Post-genomics
KW - Reduces genomes
KW - T. whipplei
UR - http://www.scopus.com/inward/record.url?scp=19944415303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19944415303&partnerID=8YFLogxK
U2 - 10.2174/1389202053971929
DO - 10.2174/1389202053971929
M3 - Review article
AN - SCOPUS:19944415303
VL - 6
SP - 195
EP - 205
JO - Current Genomics
JF - Current Genomics
SN - 1389-2029
IS - 3
ER -