Tropheryma whipplei genome at the beginning of the post-genomic era

N. Crapoulet, P. Renesto, J. S. Dumler, Karsten Suhre, H. Ogata, J. M. Claverie, Didier Raoult

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Tropheryma whipplei is a Gram positive human pathogen that is the causative agent of Whipple's disease. Nearly one century elapsed between the first description of the disease in 1907 and the cultivation of this bacterium within eukaryotic cell cultures in 2000. This achievement has made possible genome sequencing of this poorly studied microorganism. This review summarizes post-genomic knowledge resulting from these genomic data. To compare the theoretical genetic capabilities of T. whipplei with those of other sequenced bacteria, a virtual microarray representation was generated. This in silico analysis supports the concept of independent evolution pathways for microbial pathogens. Concrete post-genomic consequences related to clinical microbiology such as the analysis of antibiotic susceptibility or the design of molecular tools convenient for PCR detection and epidemiology studies are described. Analysis of whole-cell metabolic networks of T. whipplei also provide clues for designing axenic media for this pathogen that is particularly recalcitrant to cultivation. This opens the way to investigate transcriptome analysis of T. whipplei by microarrays. Future prospects are also discussed.

Original languageEnglish
Pages (from-to)195-205
Number of pages11
JournalCurrent Genomics
Volume6
Issue number3
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Tropheryma
Genome
Whipple Disease
Bacteria
Gene Expression Profiling
Eukaryotic Cells
Metabolic Networks and Pathways
Microbiology
Computer Simulation
Epidemiology
Cell Culture Techniques
Anti-Bacterial Agents
Polymerase Chain Reaction

Keywords

  • Post-genomics
  • Reduces genomes
  • T. whipplei

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Crapoulet, N., Renesto, P., Dumler, J. S., Suhre, K., Ogata, H., Claverie, J. M., & Raoult, D. (2005). Tropheryma whipplei genome at the beginning of the post-genomic era. Current Genomics, 6(3), 195-205. https://doi.org/10.2174/1389202053971929

Tropheryma whipplei genome at the beginning of the post-genomic era. / Crapoulet, N.; Renesto, P.; Dumler, J. S.; Suhre, Karsten; Ogata, H.; Claverie, J. M.; Raoult, Didier.

In: Current Genomics, Vol. 6, No. 3, 2005, p. 195-205.

Research output: Contribution to journalReview article

Crapoulet, N, Renesto, P, Dumler, JS, Suhre, K, Ogata, H, Claverie, JM & Raoult, D 2005, 'Tropheryma whipplei genome at the beginning of the post-genomic era', Current Genomics, vol. 6, no. 3, pp. 195-205. https://doi.org/10.2174/1389202053971929
Crapoulet, N. ; Renesto, P. ; Dumler, J. S. ; Suhre, Karsten ; Ogata, H. ; Claverie, J. M. ; Raoult, Didier. / Tropheryma whipplei genome at the beginning of the post-genomic era. In: Current Genomics. 2005 ; Vol. 6, No. 3. pp. 195-205.
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