TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites

Ali Sultan, Vandana Thathy, Ute Frevert, Kathryn J H Robson, Andrea Crisanti, Victor Nussenzweig, Ruth S. Nussenzweig, Robert Ménard

Research output: Contribution to journalArticle

457 Citations (Scopus)

Abstract

Many protozoans of the phylum Apicomplexa are invasive parasites that exhibit a substrate-dependent gliding motility. Plasmodium (malaria) sporozoites, the stage of the parasite that invades the salivary glands of the mosquito vector and the liver of the vertebrate host, express a surface protein called thrombospondin-related anonymous protein (TRAP) that has homologs in other Apicomplexa. By gene targeting in a rodent Plasmodium, we demonstrate that TRAP is critical for sporozoite infection of the mosquito salivary glands and the rat liver, and is essential for sporozoite gliding motility in vitro. This suggests that in Plasmodium sporozoites, and likely in other Apicomplexa, gliding locomotion and cell invasion have a common molecular basis.

Original languageEnglish
Pages (from-to)511-522
Number of pages12
JournalCell
Volume90
Issue number3
DOIs
Publication statusPublished - 8 Aug 1997
Externally publishedYes

Fingerprint

Thrombospondins
Sporozoites
Plasmodium
Apicomplexa
Liver
Salivary Glands
Rats
Membrane Proteins
Parasites
Proteins
Genes
Plasmodium malariae
Gene Targeting
Substrates
Culicidae
Cell Movement
Vertebrates
Rodentia
Infection

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Sultan, A., Thathy, V., Frevert, U., Robson, K. J. H., Crisanti, A., Nussenzweig, V., ... Ménard, R. (1997). TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites. Cell, 90(3), 511-522. https://doi.org/10.1016/S0092-8674(00)80511-5

TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites. / Sultan, Ali; Thathy, Vandana; Frevert, Ute; Robson, Kathryn J H; Crisanti, Andrea; Nussenzweig, Victor; Nussenzweig, Ruth S.; Ménard, Robert.

In: Cell, Vol. 90, No. 3, 08.08.1997, p. 511-522.

Research output: Contribution to journalArticle

Sultan, A, Thathy, V, Frevert, U, Robson, KJH, Crisanti, A, Nussenzweig, V, Nussenzweig, RS & Ménard, R 1997, 'TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites', Cell, vol. 90, no. 3, pp. 511-522. https://doi.org/10.1016/S0092-8674(00)80511-5
Sultan A, Thathy V, Frevert U, Robson KJH, Crisanti A, Nussenzweig V et al. TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites. Cell. 1997 Aug 8;90(3):511-522. https://doi.org/10.1016/S0092-8674(00)80511-5
Sultan, Ali ; Thathy, Vandana ; Frevert, Ute ; Robson, Kathryn J H ; Crisanti, Andrea ; Nussenzweig, Victor ; Nussenzweig, Ruth S. ; Ménard, Robert. / TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites. In: Cell. 1997 ; Vol. 90, No. 3. pp. 511-522.
@article{72b7ecdd38154d10960e229b5e3c16f4,
title = "TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites",
abstract = "Many protozoans of the phylum Apicomplexa are invasive parasites that exhibit a substrate-dependent gliding motility. Plasmodium (malaria) sporozoites, the stage of the parasite that invades the salivary glands of the mosquito vector and the liver of the vertebrate host, express a surface protein called thrombospondin-related anonymous protein (TRAP) that has homologs in other Apicomplexa. By gene targeting in a rodent Plasmodium, we demonstrate that TRAP is critical for sporozoite infection of the mosquito salivary glands and the rat liver, and is essential for sporozoite gliding motility in vitro. This suggests that in Plasmodium sporozoites, and likely in other Apicomplexa, gliding locomotion and cell invasion have a common molecular basis.",
author = "Ali Sultan and Vandana Thathy and Ute Frevert and Robson, {Kathryn J H} and Andrea Crisanti and Victor Nussenzweig and Nussenzweig, {Ruth S.} and Robert M{\'e}nard",
year = "1997",
month = "8",
day = "8",
doi = "10.1016/S0092-8674(00)80511-5",
language = "English",
volume = "90",
pages = "511--522",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites

AU - Sultan, Ali

AU - Thathy, Vandana

AU - Frevert, Ute

AU - Robson, Kathryn J H

AU - Crisanti, Andrea

AU - Nussenzweig, Victor

AU - Nussenzweig, Ruth S.

AU - Ménard, Robert

PY - 1997/8/8

Y1 - 1997/8/8

N2 - Many protozoans of the phylum Apicomplexa are invasive parasites that exhibit a substrate-dependent gliding motility. Plasmodium (malaria) sporozoites, the stage of the parasite that invades the salivary glands of the mosquito vector and the liver of the vertebrate host, express a surface protein called thrombospondin-related anonymous protein (TRAP) that has homologs in other Apicomplexa. By gene targeting in a rodent Plasmodium, we demonstrate that TRAP is critical for sporozoite infection of the mosquito salivary glands and the rat liver, and is essential for sporozoite gliding motility in vitro. This suggests that in Plasmodium sporozoites, and likely in other Apicomplexa, gliding locomotion and cell invasion have a common molecular basis.

AB - Many protozoans of the phylum Apicomplexa are invasive parasites that exhibit a substrate-dependent gliding motility. Plasmodium (malaria) sporozoites, the stage of the parasite that invades the salivary glands of the mosquito vector and the liver of the vertebrate host, express a surface protein called thrombospondin-related anonymous protein (TRAP) that has homologs in other Apicomplexa. By gene targeting in a rodent Plasmodium, we demonstrate that TRAP is critical for sporozoite infection of the mosquito salivary glands and the rat liver, and is essential for sporozoite gliding motility in vitro. This suggests that in Plasmodium sporozoites, and likely in other Apicomplexa, gliding locomotion and cell invasion have a common molecular basis.

UR - http://www.scopus.com/inward/record.url?scp=0030745648&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030745648&partnerID=8YFLogxK

U2 - 10.1016/S0092-8674(00)80511-5

DO - 10.1016/S0092-8674(00)80511-5

M3 - Article

VL - 90

SP - 511

EP - 522

JO - Cell

JF - Cell

SN - 0092-8674

IS - 3

ER -