Transportin 2 regulates apoptosis through the RNA-binding protein HuR

Christopher Von Roretz, Angelo M. Macri, Imed Gallouzi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In response to severe stress, apoptotic cell death is engaged. Apoptosis is a well orchestrated process that involves the activation and implication of many factors. In this study, we identified a role for the nuclear trafficking factorTRN2(transportin 2) in cell death. TRN2 is normally responsible for the nuclear import of the RNA-binding protein HuR. During apoptosis, however, HuR accumulates in the cytoplasm. This is due to the caspase-mediated cleavage of the cytoplasmic fraction of HuR. One of the cleavage fragments generated by this processing of HuR interacts with TRN2 and thus blocks the re-import of HuR into the nucleus. This concentrates HuR in the cytoplasm, advancing apoptosis. Therefore, increasing or decreasing the levels of TRN2 has an inverse consequential effect on cell death, demonstrating for the first time the role of a nucleocytoplasmic transport factor in apoptosis.

Original languageEnglish
Pages (from-to)25983-25991
Number of pages9
JournalJournal of Biological Chemistry
Volume286
Issue number29
DOIs
Publication statusPublished - 22 Jul 2011
Externally publishedYes

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Karyopherins
RNA-Binding Proteins
Cell death
Apoptosis
Cell Death
Cell Nucleus Active Transport
Cytoplasm
Nuclear RNA
Caspases
Chemical activation
Processing

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Transportin 2 regulates apoptosis through the RNA-binding protein HuR. / Von Roretz, Christopher; Macri, Angelo M.; Gallouzi, Imed.

In: Journal of Biological Chemistry, Vol. 286, No. 29, 22.07.2011, p. 25983-25991.

Research output: Contribution to journalArticle

Von Roretz, Christopher ; Macri, Angelo M. ; Gallouzi, Imed. / Transportin 2 regulates apoptosis through the RNA-binding protein HuR. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 29. pp. 25983-25991.
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