Transmembrane signalling via the T cell antigen receptor heterodimer and the CD2 antigen: A synergistic pathway for activation of T cells

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

T cell surface proteins involved in transmembrane signalling resulting in the activation of T cells were investigated utilizing as probes monoclonal antibodies directed at T cell surface antigens. Here we report that mAbs that react with a framework determinant of α/β heterodimer of T cell receptor for antigen, anti-TCR-1, and those with the SRBC-binding epitope of the CD2 antigen, OKT11, are synergistic in promoting T cell proliferation. The proliferative response is dependent upon crosslinking of anti-TCR-1 and OKT11, and is associated with a significant increase in the concentration of intracellular free calcium in T cells. Moreover, EGTA and a direct (staurosporine) or a competitive (1- [5-isoquinolinylsulfonyl]-2-methyl piperazine) inhibitor of protein kinase C prevents T cell proliferation accomplished with crosslinked anti-TCR-1 and OKT11. Our findings, in addition to demonstrating the synergism between the signals initiated via the T cell receptor for antigen and the CD2 antigen, suggest a role for calcium and protein kinase C in the transduction of signals generated with crosslinked anti-TCR-1 and OKT11.

Original languageEnglish
Pages (from-to)348-351
Number of pages4
JournalTransplantation
Volume47
Issue number2
Publication statusPublished - 1 Jan 1989
Externally publishedYes

Fingerprint

CD2 Antigens
T-Cell Antigen Receptor
T-Lymphocytes
Protein Kinase C
Cell Proliferation
Calcium
Staurosporine
Egtazic Acid
Surface Antigens
Epitopes
Signal Transduction
Membrane Proteins
Monoclonal Antibodies

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

@article{164fe0f64abb463ca8653c8a688f94d4,
title = "Transmembrane signalling via the T cell antigen receptor heterodimer and the CD2 antigen: A synergistic pathway for activation of T cells",
abstract = "T cell surface proteins involved in transmembrane signalling resulting in the activation of T cells were investigated utilizing as probes monoclonal antibodies directed at T cell surface antigens. Here we report that mAbs that react with a framework determinant of α/β heterodimer of T cell receptor for antigen, anti-TCR-1, and those with the SRBC-binding epitope of the CD2 antigen, OKT11, are synergistic in promoting T cell proliferation. The proliferative response is dependent upon crosslinking of anti-TCR-1 and OKT11, and is associated with a significant increase in the concentration of intracellular free calcium in T cells. Moreover, EGTA and a direct (staurosporine) or a competitive (1- [5-isoquinolinylsulfonyl]-2-methyl piperazine) inhibitor of protein kinase C prevents T cell proliferation accomplished with crosslinked anti-TCR-1 and OKT11. Our findings, in addition to demonstrating the synergism between the signals initiated via the T cell receptor for antigen and the CD2 antigen, suggest a role for calcium and protein kinase C in the transduction of signals generated with crosslinked anti-TCR-1 and OKT11.",
author = "Manikkam Suthanthiran",
year = "1989",
month = "1",
day = "1",
language = "English",
volume = "47",
pages = "348--351",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Transmembrane signalling via the T cell antigen receptor heterodimer and the CD2 antigen

T2 - A synergistic pathway for activation of T cells

AU - Suthanthiran, Manikkam

PY - 1989/1/1

Y1 - 1989/1/1

N2 - T cell surface proteins involved in transmembrane signalling resulting in the activation of T cells were investigated utilizing as probes monoclonal antibodies directed at T cell surface antigens. Here we report that mAbs that react with a framework determinant of α/β heterodimer of T cell receptor for antigen, anti-TCR-1, and those with the SRBC-binding epitope of the CD2 antigen, OKT11, are synergistic in promoting T cell proliferation. The proliferative response is dependent upon crosslinking of anti-TCR-1 and OKT11, and is associated with a significant increase in the concentration of intracellular free calcium in T cells. Moreover, EGTA and a direct (staurosporine) or a competitive (1- [5-isoquinolinylsulfonyl]-2-methyl piperazine) inhibitor of protein kinase C prevents T cell proliferation accomplished with crosslinked anti-TCR-1 and OKT11. Our findings, in addition to demonstrating the synergism between the signals initiated via the T cell receptor for antigen and the CD2 antigen, suggest a role for calcium and protein kinase C in the transduction of signals generated with crosslinked anti-TCR-1 and OKT11.

AB - T cell surface proteins involved in transmembrane signalling resulting in the activation of T cells were investigated utilizing as probes monoclonal antibodies directed at T cell surface antigens. Here we report that mAbs that react with a framework determinant of α/β heterodimer of T cell receptor for antigen, anti-TCR-1, and those with the SRBC-binding epitope of the CD2 antigen, OKT11, are synergistic in promoting T cell proliferation. The proliferative response is dependent upon crosslinking of anti-TCR-1 and OKT11, and is associated with a significant increase in the concentration of intracellular free calcium in T cells. Moreover, EGTA and a direct (staurosporine) or a competitive (1- [5-isoquinolinylsulfonyl]-2-methyl piperazine) inhibitor of protein kinase C prevents T cell proliferation accomplished with crosslinked anti-TCR-1 and OKT11. Our findings, in addition to demonstrating the synergism between the signals initiated via the T cell receptor for antigen and the CD2 antigen, suggest a role for calcium and protein kinase C in the transduction of signals generated with crosslinked anti-TCR-1 and OKT11.

UR - http://www.scopus.com/inward/record.url?scp=0024520892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024520892&partnerID=8YFLogxK

M3 - Article

C2 - 2563920

AN - SCOPUS:0024520892

VL - 47

SP - 348

EP - 351

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 2

ER -