Transcriptional profile of postmortem skeletal muscle

Despina Sanoudou, Peter B. Kang, Judith N. Haslett, Mei Han, Louis M. Kunkel, Alan H. Beggs

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Autopsy specimens are often used in molecular biological studies of disease pathophysiology. However, few analyses have focused specifically on postmortem changes in skeletal muscles, and almost all of those investigate protein or metabolic changes. Although some structural and enzymatic changes have been described, the sequence of transcriptional events associated with these remains unclear. We analyzed a series of new and preexisting human skeletal muscle data sets on ≃12,500 genes and expressed sequence tags (ESTs) generated by the Affymetrix U95Av2 GeneChips from seven autopsy and seven surgical specimens. Remarkably, postmortem specimens (up to 46 h) revealed a significant and prominent upregulation of transcripts involved with protein biosynthesis. Additional upregulated transcripts are associated with cellular responses to oxidative stress, hypoxia, and ischemia; however, only a subset of genes in these pathways was affected. Overexpression was also seen for apoptosis-related, cell cycle regulation/arrest-related, and signal transduction-related genes. No major gene expression differences were seen between autopsy specimens with <20-h and 34- to 46-h postmortem intervals or between pediatric and adult cases. These data demonstrate that, likely in response to hypoxia and oxidative stress, skeletal muscle undergoes a highly active transcriptional, and possibly, translational phase during the initial 46-h postmortem interval. Knowledge of these changes is important for proper interpretation of gene expression studies utilizing autopsy specimens.

Original languageEnglish
Pages (from-to)222-228
Number of pages7
JournalPhysiological Genomics
Volume16
DOIs
Publication statusPublished - 1 Apr 2004
Externally publishedYes

Fingerprint

Autopsy
Skeletal Muscle
Oxidative Stress
Postmortem Changes
Genes
Gene Expression
Expressed Sequence Tags
Protein Biosynthesis
Cell Cycle Checkpoints
Signal Transduction
Up-Regulation
Ischemia
Pediatrics
Apoptosis
Proteins
Hypoxia

Keywords

  • Autopsy
  • Gene expression
  • Microarrays
  • Surgical

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Sanoudou, D., Kang, P. B., Haslett, J. N., Han, M., Kunkel, L. M., & Beggs, A. H. (2004). Transcriptional profile of postmortem skeletal muscle. Physiological Genomics, 16, 222-228. https://doi.org/10.1152/physiolgenomics.00137.2003

Transcriptional profile of postmortem skeletal muscle. / Sanoudou, Despina; Kang, Peter B.; Haslett, Judith N.; Han, Mei; Kunkel, Louis M.; Beggs, Alan H.

In: Physiological Genomics, Vol. 16, 01.04.2004, p. 222-228.

Research output: Contribution to journalArticle

Sanoudou, D, Kang, PB, Haslett, JN, Han, M, Kunkel, LM & Beggs, AH 2004, 'Transcriptional profile of postmortem skeletal muscle', Physiological Genomics, vol. 16, pp. 222-228. https://doi.org/10.1152/physiolgenomics.00137.2003
Sanoudou D, Kang PB, Haslett JN, Han M, Kunkel LM, Beggs AH. Transcriptional profile of postmortem skeletal muscle. Physiological Genomics. 2004 Apr 1;16:222-228. https://doi.org/10.1152/physiolgenomics.00137.2003
Sanoudou, Despina ; Kang, Peter B. ; Haslett, Judith N. ; Han, Mei ; Kunkel, Louis M. ; Beggs, Alan H. / Transcriptional profile of postmortem skeletal muscle. In: Physiological Genomics. 2004 ; Vol. 16. pp. 222-228.
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