Transcriptional activation of the CEF-4/9E3 cytokine gene by pp60v-src

Mohammed Dehbi, Angélique Mbiguino, Myriam Beauchemin, Gilles Chatelain, Pierre André Bédard

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Abstract

The CEF-4/9E3 gene is expressed constitutively in Rous sarcoma virus (RSV)-transformed cells. This expression is largely determined by an increase in transcription of the gene. In this report, we characterize the regulatory elements responsible for the transformation-dependent activation of CEF-4/9E3. Three sequences corresponding to AP-1, PRD II/κB, and TAACGCAATT are involved in the process and therefore define the src-responsive unit (SRU) of the CEF-4 promoter. In constructs containing a deletion of the SRU, multiple copies of AP-1 or PRD II/κB, but not TAACGCAATT, led to activation of the promoter. Thus, factors interacting with these elements are constitutively activated in RSV-transformed chicken embryo fibroblasts. In agreement with the results of transient expression assays, proteins binding to AP-1, PRD II/κB, and TAACGCAATT were more abundant in the nuclei of transformed cells. The expression of the CEF-4 promoter was investigated in cells infected by a temperature-sensitive mutant of RSV. No significant increase in CEF-4 promoter activity was detected early after activation of pp60v-src. In contrast, a substantial activation of the CEF-4 promoter was detected late after a temperature shift. Factors interacting with the TAACGCAATT, PRD II/κB, and AP-1 elements accumulated gradually over a period of several hours. Therefore, transcriptional activation plays an important role in the late, constitutive expression of the CEF-4 gene in stably transformed cells.

Original languageEnglish
Pages (from-to)1490-1499
Number of pages10
JournalMolecular and Cellular Biology
Volume12
Issue number4
Publication statusPublished - 1 Apr 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Dehbi, M., Mbiguino, A., Beauchemin, M., Chatelain, G., & Bédard, P. A. (1992). Transcriptional activation of the CEF-4/9E3 cytokine gene by pp60v-src Molecular and Cellular Biology, 12(4), 1490-1499.