Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo

Atfa Sassi, Beda Brichacek, Sara Hieny, Felix Yarovinsky, Hana Golding, Jean-Charles B. Grivel, Alan Sher, Leonid Margolis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Critical events of HIV-1 pathogenesis occur in lymphoid tissues where HIV-1 is typically accompanied by infections with other pathogens (HIV co-pathogens). Co-pathogens greatly affect the clinical course of the disease and the transmission of HIV. The apicomplexan parasite Toxoplasma gondii is a common HIV co-pathogen associated with AIDS development. Here, we examined the interaction of T. gondii and HIV in coinfected human lymphoid tissue ex vivo. Both pathogens readily replicate in ex vivo infected blocks of human tonsillar tissue. Surprisingly, we found that live T. gondii preferentially inhibits R5 HIV-1 replication in coinfected tissues. This effect is reproduced by treatment of the tissue blocks with recombinant C-18, a T. gondii-encoded cyclophilin that binds to CCR5. These ex vivo findings raise the possibility that, in addition to being a co-factor in HIV disease, T. gondii may influence the outcome of viral infection by preferentially suppressing R5 variants.

Original languageEnglish
Pages (from-to)1106-1113
Number of pages8
JournalMicrobes and Infection
Volume11
Issue number14-15
DOIs
Publication statusPublished - Dec 2009
Externally publishedYes

Fingerprint

Toxoplasma
Lymphoid Tissue
HIV-1
HIV
Cyclophilins
Virus Diseases
Parasites
Acquired Immunodeficiency Syndrome
Infection

Keywords

  • Cyclophilin
  • Ex vivo
  • HIV
  • Human lymphoid tissue
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Sassi, A., Brichacek, B., Hieny, S., Yarovinsky, F., Golding, H., Grivel, J-C. B., ... Margolis, L. (2009). Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo. Microbes and Infection, 11(14-15), 1106-1113. https://doi.org/10.1016/j.micinf.2009.08.004

Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo. / Sassi, Atfa; Brichacek, Beda; Hieny, Sara; Yarovinsky, Felix; Golding, Hana; Grivel, Jean-Charles B.; Sher, Alan; Margolis, Leonid.

In: Microbes and Infection, Vol. 11, No. 14-15, 12.2009, p. 1106-1113.

Research output: Contribution to journalArticle

Sassi, A, Brichacek, B, Hieny, S, Yarovinsky, F, Golding, H, Grivel, J-CB, Sher, A & Margolis, L 2009, 'Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo', Microbes and Infection, vol. 11, no. 14-15, pp. 1106-1113. https://doi.org/10.1016/j.micinf.2009.08.004
Sassi, Atfa ; Brichacek, Beda ; Hieny, Sara ; Yarovinsky, Felix ; Golding, Hana ; Grivel, Jean-Charles B. ; Sher, Alan ; Margolis, Leonid. / Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo. In: Microbes and Infection. 2009 ; Vol. 11, No. 14-15. pp. 1106-1113.
@article{6286c31fcba24f1c8c83c80222d283ab,
title = "Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo",
abstract = "Critical events of HIV-1 pathogenesis occur in lymphoid tissues where HIV-1 is typically accompanied by infections with other pathogens (HIV co-pathogens). Co-pathogens greatly affect the clinical course of the disease and the transmission of HIV. The apicomplexan parasite Toxoplasma gondii is a common HIV co-pathogen associated with AIDS development. Here, we examined the interaction of T. gondii and HIV in coinfected human lymphoid tissue ex vivo. Both pathogens readily replicate in ex vivo infected blocks of human tonsillar tissue. Surprisingly, we found that live T. gondii preferentially inhibits R5 HIV-1 replication in coinfected tissues. This effect is reproduced by treatment of the tissue blocks with recombinant C-18, a T. gondii-encoded cyclophilin that binds to CCR5. These ex vivo findings raise the possibility that, in addition to being a co-factor in HIV disease, T. gondii may influence the outcome of viral infection by preferentially suppressing R5 variants.",
keywords = "Cyclophilin, Ex vivo, HIV, Human lymphoid tissue, Toxoplasma gondii",
author = "Atfa Sassi and Beda Brichacek and Sara Hieny and Felix Yarovinsky and Hana Golding and Grivel, {Jean-Charles B.} and Alan Sher and Leonid Margolis",
year = "2009",
month = "12",
doi = "10.1016/j.micinf.2009.08.004",
language = "English",
volume = "11",
pages = "1106--1113",
journal = "Microbes and Infection",
issn = "1286-4579",
publisher = "Elsevier Masson SAS",
number = "14-15",

}

TY - JOUR

T1 - Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo

AU - Sassi, Atfa

AU - Brichacek, Beda

AU - Hieny, Sara

AU - Yarovinsky, Felix

AU - Golding, Hana

AU - Grivel, Jean-Charles B.

AU - Sher, Alan

AU - Margolis, Leonid

PY - 2009/12

Y1 - 2009/12

N2 - Critical events of HIV-1 pathogenesis occur in lymphoid tissues where HIV-1 is typically accompanied by infections with other pathogens (HIV co-pathogens). Co-pathogens greatly affect the clinical course of the disease and the transmission of HIV. The apicomplexan parasite Toxoplasma gondii is a common HIV co-pathogen associated with AIDS development. Here, we examined the interaction of T. gondii and HIV in coinfected human lymphoid tissue ex vivo. Both pathogens readily replicate in ex vivo infected blocks of human tonsillar tissue. Surprisingly, we found that live T. gondii preferentially inhibits R5 HIV-1 replication in coinfected tissues. This effect is reproduced by treatment of the tissue blocks with recombinant C-18, a T. gondii-encoded cyclophilin that binds to CCR5. These ex vivo findings raise the possibility that, in addition to being a co-factor in HIV disease, T. gondii may influence the outcome of viral infection by preferentially suppressing R5 variants.

AB - Critical events of HIV-1 pathogenesis occur in lymphoid tissues where HIV-1 is typically accompanied by infections with other pathogens (HIV co-pathogens). Co-pathogens greatly affect the clinical course of the disease and the transmission of HIV. The apicomplexan parasite Toxoplasma gondii is a common HIV co-pathogen associated with AIDS development. Here, we examined the interaction of T. gondii and HIV in coinfected human lymphoid tissue ex vivo. Both pathogens readily replicate in ex vivo infected blocks of human tonsillar tissue. Surprisingly, we found that live T. gondii preferentially inhibits R5 HIV-1 replication in coinfected tissues. This effect is reproduced by treatment of the tissue blocks with recombinant C-18, a T. gondii-encoded cyclophilin that binds to CCR5. These ex vivo findings raise the possibility that, in addition to being a co-factor in HIV disease, T. gondii may influence the outcome of viral infection by preferentially suppressing R5 variants.

KW - Cyclophilin

KW - Ex vivo

KW - HIV

KW - Human lymphoid tissue

KW - Toxoplasma gondii

UR - http://www.scopus.com/inward/record.url?scp=71649113049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71649113049&partnerID=8YFLogxK

U2 - 10.1016/j.micinf.2009.08.004

DO - 10.1016/j.micinf.2009.08.004

M3 - Article

VL - 11

SP - 1106

EP - 1113

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

IS - 14-15

ER -