TNF-related apoptosis-inducing ligand (TRAIL) in HIV-1-infected patients and its in vitro production by antigen-presenting cells

Jean Philippe Herbeuval, Adriano Boasso, Jean-Charles B. Grivel, Andrew W. Hardy, Stephanie A. Anderson, Matthew J. Dolan, Claire Chougnet, Jeffrey D. Lifson, Gene M. Shearer

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

There is now considerable in vitro evidence that tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is involved in HIV-1 pathogenesis by inducing CD4+ T-cell death characteristic of AIDS. Therefore, we have tested levels of TRAIL in plasma samples from 107 HIV-1-infected and 53 uninfected controls as well as in longitudinal plasma samples from patients who started antiretroviral therapy (ART). TRAIL was elevated In plasma of HIV-1-infected patients compared with uninfected individuals, and patients receiving ART showed decreased plasma TRAIL levels that correlated with reduction in viral load. In vitro exposure to infectious and noninfectious HIV-1 induced TRAIL in monocytes and marginally in dendritic cells (DCs) but not in macrophages or T cells. Interestingly, the HIV-1 entry inhibitor, soluble CD4, blocked HIV-1-induced production of TRAIL. Furthermore, production and gene expression of TRAIL by monocytes were regulated by type I interferon via signal transducer and activator of transcription-1 (STAT1)/STAT2 signaling molecule. Ex vivo HIV-1 infection of human tonsil lymphoid tissue also resulted in increased TRAIL production. We demonstrate here that plasma TRAIL is elevated in HSV-1-infected patients and is decreased by ART therapy. The high production of TRAIL by antigen-presenting cells may contribute to the death of CD4+ T cells during progression to AIDS.

Original languageEnglish
Pages (from-to)2458-2464
Number of pages7
JournalBlood
Volume105
Issue number6
DOIs
Publication statusPublished - 15 Mar 2005
Externally publishedYes

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TNF-Related Apoptosis-Inducing Ligand
Antigen-Presenting Cells
HIV-1
Tumor Necrosis Factor-alpha
Apoptosis
Ligands
T-cells
Plasmas
T-Lymphocytes
Monocytes
Acquired Immunodeficiency Syndrome
HIV Fusion Inhibitors
STAT1 Transcription Factor
In Vitro Techniques
Interferon Type I
Macrophages
Palatine Tonsil
Human Herpesvirus 1
Lymphoid Tissue
Cell death

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

Cite this

TNF-related apoptosis-inducing ligand (TRAIL) in HIV-1-infected patients and its in vitro production by antigen-presenting cells. / Herbeuval, Jean Philippe; Boasso, Adriano; Grivel, Jean-Charles B.; Hardy, Andrew W.; Anderson, Stephanie A.; Dolan, Matthew J.; Chougnet, Claire; Lifson, Jeffrey D.; Shearer, Gene M.

In: Blood, Vol. 105, No. 6, 15.03.2005, p. 2458-2464.

Research output: Contribution to journalArticle

Herbeuval, JP, Boasso, A, Grivel, J-CB, Hardy, AW, Anderson, SA, Dolan, MJ, Chougnet, C, Lifson, JD & Shearer, GM 2005, 'TNF-related apoptosis-inducing ligand (TRAIL) in HIV-1-infected patients and its in vitro production by antigen-presenting cells', Blood, vol. 105, no. 6, pp. 2458-2464. https://doi.org/10.1182/blood-2004-08-3058
Herbeuval, Jean Philippe ; Boasso, Adriano ; Grivel, Jean-Charles B. ; Hardy, Andrew W. ; Anderson, Stephanie A. ; Dolan, Matthew J. ; Chougnet, Claire ; Lifson, Jeffrey D. ; Shearer, Gene M. / TNF-related apoptosis-inducing ligand (TRAIL) in HIV-1-infected patients and its in vitro production by antigen-presenting cells. In: Blood. 2005 ; Vol. 105, No. 6. pp. 2458-2464.
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