TLR2, TLR4 and CD86 gene polymorphisms in recurrent aphthous stomatitis

Jumana Karasneh, Maisoun Bani-Hani, Asem Alkhateeb, Ahmad Hassan, Firas Alzoubi, Martin Thornhill

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Recurrent aphthous stomatitis (RAS) is an inflammatory disease induced by genetic and environmental factors. Toll-like receptor (TLR) and CD86 are essential components for innate immunity and cellular immune response. We aimed to determine whether inheritance of specific TLR2, TLR4and CD86 gene polymorphisms are associated with RAS. Methods: Ninety-six patients with RAS and 153 controls were studied. Eight SNPs were genotyped using PCR-RFLP technique; four in TLR2 gene: rs4696480, rs3804100, rs121917864, rs5743708; three in TLR4 gene: rs10759931, rs4986790 rs1927911; and one in CD86 gene rs17281995. Association was assessed by logistic regression analysis. Linkage disequilibrium (LD) was assessed using the Haploview program. Results: Significant increase in inheritance of A allele (OR = 1.6, P = 0.01) and AA genotype (OR = 3.89, P = 0.01) of TLR4 rs10759931 was observed in cases. TLR4rs1927911 C allele and CC genotype were also increased (OR = 1.60 and 2.78 respectively); however, this was not statistically significant (P = 0.02 and 0.03 respectively). TLR2 and CD86 did not show association with RAS. Conclusions: This is the first study to investigate the association of TLR and CD86 with RAS. We found a significant association between TLR4 rs10759931 polymorphism and RAS. Confirmatory studies in other populations and functional investigations are needed to determine the role of TLR4 in RAS.

Original languageEnglish
Pages (from-to)857-863
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume44
Issue number10
DOIs
Publication statusPublished - 1 Nov 2015
Externally publishedYes

Fingerprint

Genes
Toll-Like Receptors
Innate Immunity
Alleles
Genotype
Inborn Genetic Diseases
Linkage Disequilibrium
Sutton disease 2
Cellular Immunity
Restriction Fragment Length Polymorphisms
Single Nucleotide Polymorphism
Logistic Models
Regression Analysis
Polymerase Chain Reaction
Population

Keywords

  • Co-stimulatory protein
  • Polymorphism
  • Recurrent aphthous stomatitis
  • Toll-like receptor

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics

Cite this

Karasneh, J., Bani-Hani, M., Alkhateeb, A., Hassan, A., Alzoubi, F., & Thornhill, M. (2015). TLR2, TLR4 and CD86 gene polymorphisms in recurrent aphthous stomatitis. Journal of Oral Pathology and Medicine, 44(10), 857-863. https://doi.org/10.1111/jop.12298

TLR2, TLR4 and CD86 gene polymorphisms in recurrent aphthous stomatitis. / Karasneh, Jumana; Bani-Hani, Maisoun; Alkhateeb, Asem; Hassan, Ahmad; Alzoubi, Firas; Thornhill, Martin.

In: Journal of Oral Pathology and Medicine, Vol. 44, No. 10, 01.11.2015, p. 857-863.

Research output: Contribution to journalArticle

Karasneh, J, Bani-Hani, M, Alkhateeb, A, Hassan, A, Alzoubi, F & Thornhill, M 2015, 'TLR2, TLR4 and CD86 gene polymorphisms in recurrent aphthous stomatitis', Journal of Oral Pathology and Medicine, vol. 44, no. 10, pp. 857-863. https://doi.org/10.1111/jop.12298
Karasneh J, Bani-Hani M, Alkhateeb A, Hassan A, Alzoubi F, Thornhill M. TLR2, TLR4 and CD86 gene polymorphisms in recurrent aphthous stomatitis. Journal of Oral Pathology and Medicine. 2015 Nov 1;44(10):857-863. https://doi.org/10.1111/jop.12298
Karasneh, Jumana ; Bani-Hani, Maisoun ; Alkhateeb, Asem ; Hassan, Ahmad ; Alzoubi, Firas ; Thornhill, Martin. / TLR2, TLR4 and CD86 gene polymorphisms in recurrent aphthous stomatitis. In: Journal of Oral Pathology and Medicine. 2015 ; Vol. 44, No. 10. pp. 857-863.
@article{4d96655cb43f4942a4e898ec5c2d1b2a,
title = "TLR2, TLR4 and CD86 gene polymorphisms in recurrent aphthous stomatitis",
abstract = "Background: Recurrent aphthous stomatitis (RAS) is an inflammatory disease induced by genetic and environmental factors. Toll-like receptor (TLR) and CD86 are essential components for innate immunity and cellular immune response. We aimed to determine whether inheritance of specific TLR2, TLR4and CD86 gene polymorphisms are associated with RAS. Methods: Ninety-six patients with RAS and 153 controls were studied. Eight SNPs were genotyped using PCR-RFLP technique; four in TLR2 gene: rs4696480, rs3804100, rs121917864, rs5743708; three in TLR4 gene: rs10759931, rs4986790 rs1927911; and one in CD86 gene rs17281995. Association was assessed by logistic regression analysis. Linkage disequilibrium (LD) was assessed using the Haploview program. Results: Significant increase in inheritance of A allele (OR = 1.6, P = 0.01) and AA genotype (OR = 3.89, P = 0.01) of TLR4 rs10759931 was observed in cases. TLR4rs1927911 C allele and CC genotype were also increased (OR = 1.60 and 2.78 respectively); however, this was not statistically significant (P = 0.02 and 0.03 respectively). TLR2 and CD86 did not show association with RAS. Conclusions: This is the first study to investigate the association of TLR and CD86 with RAS. We found a significant association between TLR4 rs10759931 polymorphism and RAS. Confirmatory studies in other populations and functional investigations are needed to determine the role of TLR4 in RAS.",
keywords = "Co-stimulatory protein, Polymorphism, Recurrent aphthous stomatitis, Toll-like receptor",
author = "Jumana Karasneh and Maisoun Bani-Hani and Asem Alkhateeb and Ahmad Hassan and Firas Alzoubi and Martin Thornhill",
year = "2015",
month = "11",
day = "1",
doi = "10.1111/jop.12298",
language = "English",
volume = "44",
pages = "857--863",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - TLR2, TLR4 and CD86 gene polymorphisms in recurrent aphthous stomatitis

AU - Karasneh, Jumana

AU - Bani-Hani, Maisoun

AU - Alkhateeb, Asem

AU - Hassan, Ahmad

AU - Alzoubi, Firas

AU - Thornhill, Martin

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Background: Recurrent aphthous stomatitis (RAS) is an inflammatory disease induced by genetic and environmental factors. Toll-like receptor (TLR) and CD86 are essential components for innate immunity and cellular immune response. We aimed to determine whether inheritance of specific TLR2, TLR4and CD86 gene polymorphisms are associated with RAS. Methods: Ninety-six patients with RAS and 153 controls were studied. Eight SNPs were genotyped using PCR-RFLP technique; four in TLR2 gene: rs4696480, rs3804100, rs121917864, rs5743708; three in TLR4 gene: rs10759931, rs4986790 rs1927911; and one in CD86 gene rs17281995. Association was assessed by logistic regression analysis. Linkage disequilibrium (LD) was assessed using the Haploview program. Results: Significant increase in inheritance of A allele (OR = 1.6, P = 0.01) and AA genotype (OR = 3.89, P = 0.01) of TLR4 rs10759931 was observed in cases. TLR4rs1927911 C allele and CC genotype were also increased (OR = 1.60 and 2.78 respectively); however, this was not statistically significant (P = 0.02 and 0.03 respectively). TLR2 and CD86 did not show association with RAS. Conclusions: This is the first study to investigate the association of TLR and CD86 with RAS. We found a significant association between TLR4 rs10759931 polymorphism and RAS. Confirmatory studies in other populations and functional investigations are needed to determine the role of TLR4 in RAS.

AB - Background: Recurrent aphthous stomatitis (RAS) is an inflammatory disease induced by genetic and environmental factors. Toll-like receptor (TLR) and CD86 are essential components for innate immunity and cellular immune response. We aimed to determine whether inheritance of specific TLR2, TLR4and CD86 gene polymorphisms are associated with RAS. Methods: Ninety-six patients with RAS and 153 controls were studied. Eight SNPs were genotyped using PCR-RFLP technique; four in TLR2 gene: rs4696480, rs3804100, rs121917864, rs5743708; three in TLR4 gene: rs10759931, rs4986790 rs1927911; and one in CD86 gene rs17281995. Association was assessed by logistic regression analysis. Linkage disequilibrium (LD) was assessed using the Haploview program. Results: Significant increase in inheritance of A allele (OR = 1.6, P = 0.01) and AA genotype (OR = 3.89, P = 0.01) of TLR4 rs10759931 was observed in cases. TLR4rs1927911 C allele and CC genotype were also increased (OR = 1.60 and 2.78 respectively); however, this was not statistically significant (P = 0.02 and 0.03 respectively). TLR2 and CD86 did not show association with RAS. Conclusions: This is the first study to investigate the association of TLR and CD86 with RAS. We found a significant association between TLR4 rs10759931 polymorphism and RAS. Confirmatory studies in other populations and functional investigations are needed to determine the role of TLR4 in RAS.

KW - Co-stimulatory protein

KW - Polymorphism

KW - Recurrent aphthous stomatitis

KW - Toll-like receptor

UR - http://www.scopus.com/inward/record.url?scp=84947042031&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947042031&partnerID=8YFLogxK

U2 - 10.1111/jop.12298

DO - 10.1111/jop.12298

M3 - Article

VL - 44

SP - 857

EP - 863

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 10

ER -