Timing and intensity of exposure to interferon-γ critically determines the function of monocyte-derived dendritic cells

Sid P. Kerkar, Dhanalakshmi Chinnasamy, Neima Hadi, Jan Melenhorst, Pawel Muranski, Alexandros Spyridonidis, Sawa Ito, Gerrit Weber, Fang Yin, Nancy Hensel, Ena Wang, Francesco M. Marincola, Austin John Barrett

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A growing body of evidence suggests that inflammatory cytokines have a dualistic role in immunity. In this study, we sought to determine the direct effects of interferon-γ (IFN-γ) on the differentiation and maturation of human peripheral blood monocyte-derived dendritic cells (moDC). Here, we report that following differentiation of monocytes into moDC with granulocyte-macrophage colony-stimulating factor and interleukin-4, IFN-γ induces moDC maturation and up-regulates the co-stimulatory markers CD80/CD86/CD95 and MHC Class I, enabling moDC to effectively generate antigen-specific CD4+ and CD8+ T-cell responses for multiple viral and tumour antigens. Early exposure of monocytes to high concentrations of IFN-γ during differentiation promotes the formation of macrophages. However, under low concentrations of IFN-γ, monocytes continue to differentiate into dendritic cells possessing a unique gene-expression profile, resulting in impairments in subsequent maturation by IFN-γ or lipopolysaccharide and an inability to generate effective antigen-specific CD4+ and CD8+ T-cell responses. These findings demonstrate that IFN-γ imparts differential programmes on moDC that shape the antigen-specific T-cell responses they induce. Timing and intensity of exposure to IFN-γ can therefore determine the functional capacity of moDC.

Original languageEnglish
Pages (from-to)96-108
Number of pages13
JournalImmunology
Volume143
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Dendritic Cells
Interferons
Monocytes
CD8 Antigens
CD4 Antigens
T-Lymphocytes
Cell Shape
Viral Tumor Antigens
Granulocyte-Macrophage Colony-Stimulating Factor
Transcriptome
Interleukin-4
Lipopolysaccharides
Immunity
Up-Regulation
Macrophages
Cytokines
Antigens

Keywords

  • Antigen presentation
  • Dendritic cells
  • Inflammation
  • Interferon-γ
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Kerkar, S. P., Chinnasamy, D., Hadi, N., Melenhorst, J., Muranski, P., Spyridonidis, A., ... Barrett, A. J. (2014). Timing and intensity of exposure to interferon-γ critically determines the function of monocyte-derived dendritic cells. Immunology, 143(1), 96-108. https://doi.org/10.1111/imm.12292

Timing and intensity of exposure to interferon-γ critically determines the function of monocyte-derived dendritic cells. / Kerkar, Sid P.; Chinnasamy, Dhanalakshmi; Hadi, Neima; Melenhorst, Jan; Muranski, Pawel; Spyridonidis, Alexandros; Ito, Sawa; Weber, Gerrit; Yin, Fang; Hensel, Nancy; Wang, Ena; Marincola, Francesco M.; Barrett, Austin John.

In: Immunology, Vol. 143, No. 1, 2014, p. 96-108.

Research output: Contribution to journalArticle

Kerkar, SP, Chinnasamy, D, Hadi, N, Melenhorst, J, Muranski, P, Spyridonidis, A, Ito, S, Weber, G, Yin, F, Hensel, N, Wang, E, Marincola, FM & Barrett, AJ 2014, 'Timing and intensity of exposure to interferon-γ critically determines the function of monocyte-derived dendritic cells', Immunology, vol. 143, no. 1, pp. 96-108. https://doi.org/10.1111/imm.12292
Kerkar, Sid P. ; Chinnasamy, Dhanalakshmi ; Hadi, Neima ; Melenhorst, Jan ; Muranski, Pawel ; Spyridonidis, Alexandros ; Ito, Sawa ; Weber, Gerrit ; Yin, Fang ; Hensel, Nancy ; Wang, Ena ; Marincola, Francesco M. ; Barrett, Austin John. / Timing and intensity of exposure to interferon-γ critically determines the function of monocyte-derived dendritic cells. In: Immunology. 2014 ; Vol. 143, No. 1. pp. 96-108.
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