Time-lapse phase-contrast microscopy fibroblast automated tracking

V. Paduano, L. Sepe, C. Cantarella, C. Sansone, G. Paolella, Michele Ceccarelli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

High-throughput applications on time-lapse microscopy allows us to follow the in vitro temporal and spatial evolution of cell populations; analysis on those kind of data will reveal cell motion parameters such as average speed, persistence and directionality, important informations for many research and therapeutic applications such as drug development or wound healing. The large quantity of frames usually acquired containing multiple cells require automated analysis methods dealing with a cell tracking process. In this work an either semi- or fully automated cell tracking system is proposed, developed to deal especially with time-lapse phase-contrast microscopy of fibroblasts, which are difficult to follow cells in some cases also for a human expert.

Original languageEnglish
Title of host publication2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010 - Proceedings
Pages284-289
Number of pages6
DOIs
Publication statusPublished - 18 Oct 2010
Externally publishedYes
Event2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010 - Thessaloniki, Greece
Duration: 1 Jul 20102 Jul 2010

Other

Other2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010
CountryGreece
CityThessaloniki
Period1/7/102/7/10

Fingerprint

Phase-Contrast Microscopy
Fibroblasts
Microscopic examination
Cell Tracking
Cells
Throughput
Therapeutic Human Experimentation
Wound Healing
Microscopy
Pharmaceutical Preparations
Population

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Radiology Nuclear Medicine and imaging

Cite this

Paduano, V., Sepe, L., Cantarella, C., Sansone, C., Paolella, G., & Ceccarelli, M. (2010). Time-lapse phase-contrast microscopy fibroblast automated tracking. In 2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010 - Proceedings (pp. 284-289). [5548533] https://doi.org/10.1109/IST.2010.5548533

Time-lapse phase-contrast microscopy fibroblast automated tracking. / Paduano, V.; Sepe, L.; Cantarella, C.; Sansone, C.; Paolella, G.; Ceccarelli, Michele.

2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010 - Proceedings. 2010. p. 284-289 5548533.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Paduano, V, Sepe, L, Cantarella, C, Sansone, C, Paolella, G & Ceccarelli, M 2010, Time-lapse phase-contrast microscopy fibroblast automated tracking. in 2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010 - Proceedings., 5548533, pp. 284-289, 2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010, Thessaloniki, Greece, 1/7/10. https://doi.org/10.1109/IST.2010.5548533
Paduano V, Sepe L, Cantarella C, Sansone C, Paolella G, Ceccarelli M. Time-lapse phase-contrast microscopy fibroblast automated tracking. In 2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010 - Proceedings. 2010. p. 284-289. 5548533 https://doi.org/10.1109/IST.2010.5548533
Paduano, V. ; Sepe, L. ; Cantarella, C. ; Sansone, C. ; Paolella, G. ; Ceccarelli, Michele. / Time-lapse phase-contrast microscopy fibroblast automated tracking. 2010 IEEE International Conference on Imaging Systems and Techniques, IST 2010 - Proceedings. 2010. pp. 284-289
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