Threshold of biologic responses of the small airway epithelium to low levels of tobacco smoke

Yael Strulovici-Barel, Larsson Omberg, Michael O'Mahony, Cynthia Gordon, Charleen Hollmann, Ann E. Tilley, Jacqueline Salit, Jason Mezey, Ben Gary Harvey, Ronald Crystal

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Rationale: Epidemiologic data demonstrate that individuals exposed to low levels of tobacco smoke have decrements in lung function and higher risk for lung disease compared with unexposed individuals. Although this risk is small, low-level tobacco smoke exposure is so widespread, it is a significant public health concern. Objectives: To identify biologic correlates of this risk we hypothesized that, compared with unexposed individuals, individuals exposed to low levels of tobacco smoke have biologic changes in the small airway epithelium, the site of the first abnormalities associated with smoking. Methods: Small airway epithelium was obtained by bronchoscopy from 121 individuals; microarrays were used to assess genome-wide gene expression; urine nicotine and cotinine were used to categorize subjects as "nonsmokers, " "active smokers," and "low exposure." Gene expression data were used to determine the threshold and induction half maximal level (ID50) of urine nicotine and cotinine at which the small airway epithelium showed abnormal responses. Measurements and Main Results: There was no threshold of urine nicotine without a small airway epithelial response, and only slightly above detectable urine cotinine threshold with a small airway epithelium response. The ID50 for nicotine was 25 ng/ml and for cotinine it was 104 ng/ml. Conclusions: The small airway epithelium detects and responds to low levels of tobacco smoke with transcriptome modifications. This provides biologic correlates of epidemiologic studies linking low-level tobacco smoke exposure to lung health risk, identifies the genes most sensitive to tobacco smoke, and defines thresholds at which the lung epithelium responds to low levels of tobacco smoke.

Original languageEnglish
Pages (from-to)1524-1532
Number of pages9
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume182
Issue number12
DOIs
Publication statusPublished - 15 Dec 2010
Externally publishedYes

Fingerprint

Smoke
Tobacco
Epithelium
Cotinine
Nicotine
Urine
Lung
Gene Expression
Bronchoscopy
Transcriptome
Lung Diseases
Epidemiologic Studies
Public Health
Smoking
Genome
Health
Genes

Keywords

  • Dose-dependant
  • Exposure
  • ID
  • Threshold

ASJC Scopus subject areas

  • Medicine(all)
  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Threshold of biologic responses of the small airway epithelium to low levels of tobacco smoke. / Strulovici-Barel, Yael; Omberg, Larsson; O'Mahony, Michael; Gordon, Cynthia; Hollmann, Charleen; Tilley, Ann E.; Salit, Jacqueline; Mezey, Jason; Harvey, Ben Gary; Crystal, Ronald.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 182, No. 12, 15.12.2010, p. 1524-1532.

Research output: Contribution to journalArticle

Strulovici-Barel, Y, Omberg, L, O'Mahony, M, Gordon, C, Hollmann, C, Tilley, AE, Salit, J, Mezey, J, Harvey, BG & Crystal, R 2010, 'Threshold of biologic responses of the small airway epithelium to low levels of tobacco smoke', American Journal of Respiratory and Critical Care Medicine, vol. 182, no. 12, pp. 1524-1532. https://doi.org/10.1164/rccm.201002-0294OC
Strulovici-Barel, Yael ; Omberg, Larsson ; O'Mahony, Michael ; Gordon, Cynthia ; Hollmann, Charleen ; Tilley, Ann E. ; Salit, Jacqueline ; Mezey, Jason ; Harvey, Ben Gary ; Crystal, Ronald. / Threshold of biologic responses of the small airway epithelium to low levels of tobacco smoke. In: American Journal of Respiratory and Critical Care Medicine. 2010 ; Vol. 182, No. 12. pp. 1524-1532.
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