Threshold levels of gene expression of the melanoma antigen gp100 correlate with tumor cell recognition by cytotoxic T lymphocytes

Adam I. Riker, Udai S. Kammula, Monica C. Panelli, Ena Wang, Galen A. Ohnmacht, Seth M. Steinberg, Steven A. Rosenberg, Francesco M. Marincola

Research output: Contribution to journalArticle

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Abstract

The level of expression of melanoma antigens (MA) may modulate the host immunologic response. Thus, the accurate measurement of MA expression may allow proper patient selection for antigen-specific therapies and yield important information for the evaluation of clinical results. In this study, we measured the absolute levels of MA messenger ribonucleic acid (mRNA) in tumor cell lines utilizing real-time quantitative reverse transcriptase- polymerase chain reaction (qRT-PCR). mRNA levels of MART-1, gp100, tyrosinase, TRP-1 and TRP-2 melanoma differentiation antigens and MAGE-1, MAGE-3 and ESO-1 cancer testis (CT) antigens were compared in 24 early- passage (<5 passages in culture) and 12 archival melanoma cell lines. MA mRNA expression was extremely variable among cell lines, occasionally reaching levels comparable to ribosomal RNA (rRNA). gp100 and MART-1 mRNA levels correlated with protein expression measurement obtained by FACS analysis. More significantly, a threshold of gp100 mRNA expression required for T-cell stimulation and target-cell killing was identified. This threshold level corresponded to approximately 500 mRNA copies per 108 copies of rRNA. Our results suggest that the measurements of MA mRNA levels may yield useful information relevant to the interpretation of clinical outcome during antigen-specific treatments.

Original languageEnglish
Pages (from-to)818-826
Number of pages9
JournalInternational Journal of Cancer
Volume86
Issue number6
Publication statusPublished - 15 Jun 2000
Externally publishedYes

Fingerprint

gp100 Melanoma Antigen
Melanoma-Specific Antigens
Cytotoxic T-Lymphocytes
RNA
Gene Expression
Neoplasms
Antigens
Cell Line
Ribosomal RNA
Monophenol Monooxygenase
Differentiation Antigens
Testicular Neoplasms
Tumor Cell Line
Reverse Transcriptase Polymerase Chain Reaction
Patient Selection
Melanoma
T-Lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Riker, A. I., Kammula, U. S., Panelli, M. C., Wang, E., Ohnmacht, G. A., Steinberg, S. M., ... Marincola, F. M. (2000). Threshold levels of gene expression of the melanoma antigen gp100 correlate with tumor cell recognition by cytotoxic T lymphocytes. International Journal of Cancer, 86(6), 818-826.

Threshold levels of gene expression of the melanoma antigen gp100 correlate with tumor cell recognition by cytotoxic T lymphocytes. / Riker, Adam I.; Kammula, Udai S.; Panelli, Monica C.; Wang, Ena; Ohnmacht, Galen A.; Steinberg, Seth M.; Rosenberg, Steven A.; Marincola, Francesco M.

In: International Journal of Cancer, Vol. 86, No. 6, 15.06.2000, p. 818-826.

Research output: Contribution to journalArticle

Riker, AI, Kammula, US, Panelli, MC, Wang, E, Ohnmacht, GA, Steinberg, SM, Rosenberg, SA & Marincola, FM 2000, 'Threshold levels of gene expression of the melanoma antigen gp100 correlate with tumor cell recognition by cytotoxic T lymphocytes', International Journal of Cancer, vol. 86, no. 6, pp. 818-826.
Riker, Adam I. ; Kammula, Udai S. ; Panelli, Monica C. ; Wang, Ena ; Ohnmacht, Galen A. ; Steinberg, Seth M. ; Rosenberg, Steven A. ; Marincola, Francesco M. / Threshold levels of gene expression of the melanoma antigen gp100 correlate with tumor cell recognition by cytotoxic T lymphocytes. In: International Journal of Cancer. 2000 ; Vol. 86, No. 6. pp. 818-826.
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