Three-dimensional structure of the M-region (bare zone) of vertebrate striated muscle myosin filaments by single-particle analysis

Hind A. AL-Khayat, Robert W. Kensler, Edward P. Morris, John M. Squire

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The rods of anti-parallel myosin molecules overlap at the centre of bipolar myosin filaments to produce an M-region (bare zone) that is free of myosin heads. Beyond the M-region edges, myosin molecules aggregate in a parallel fashion to yield the bridge regions of the myosin filaments. Adjacent myosin filaments in striated muscle A-bands are cross-linked by the M-band. Vertebrate striated muscle myosin filaments have a 3-fold rotational symmetry around their long axes. In addition, at the centre of the M-region, there are three 2-fold axes perpendicular to the filament long axis, giving the whole filament dihedral 32-point group symmetry. Here we describe the three-dimensional structure obtained by a single-particle analysis of the M-region of myosin filaments from goldfish skeletal muscle under relaxing conditions and as viewed in negative stain. This is the first single-particle reconstruction of isolated M-regions. The resulting three-dimensional reconstruction reveals details to about 55 Å resolution of the density distribution in the five main nonmyosin densities in the M-band (M6', M4', M1, M4 and M6) and in the myosin head crowns (P1, P2 and P3) at the M-region edges. The outermost crowns in the reconstruction were identified specifically by their close similarity to the corresponding crown levels in our previously published bridge region reconstructions. The packing of myosin molecules into the M-region structure is discussed, and some unidentified densities are highlighted.

Original languageEnglish
Pages (from-to)763-776
Number of pages14
JournalJournal of Molecular Biology
Volume403
Issue number5
DOIs
Publication statusPublished - 12 Nov 2010
Externally publishedYes

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Striated Muscle
Myosins
Vertebrates
Crowns
Goldfish
Skeletal Muscle
Coloring Agents

Keywords

  • 3D reconstruction
  • Bare zone region
  • Fish skeletal muscle
  • M-region
  • Myosin filament

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Three-dimensional structure of the M-region (bare zone) of vertebrate striated muscle myosin filaments by single-particle analysis. / AL-Khayat, Hind A.; Kensler, Robert W.; Morris, Edward P.; Squire, John M.

In: Journal of Molecular Biology, Vol. 403, No. 5, 12.11.2010, p. 763-776.

Research output: Contribution to journalArticle

AL-Khayat, Hind A. ; Kensler, Robert W. ; Morris, Edward P. ; Squire, John M. / Three-dimensional structure of the M-region (bare zone) of vertebrate striated muscle myosin filaments by single-particle analysis. In: Journal of Molecular Biology. 2010 ; Vol. 403, No. 5. pp. 763-776.
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