Three-dimensional organization of block copolymers on "DNA- minimal" scaffolds

Christopher K. McLaughlin, Graham D. Hamblin, Kevin D. Hänni, Justin W. Conway, Manoj K. Nayak, Karina M.M. Carneiro, Hassan S. Bazzi, Hanadi F. Sleiman

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Here, we introduce a 3D-DNA construction method that assembles a minimum number of DNA strands in quantitative yield, to give a scaffold with a large number of single-stranded arms. This DNA frame is used as a core structure to organize other functional materials in 3D as the shell. We use the ring-opening metathesis polymerization (ROMP) to generate block copolymers that are covalently attached to DNA strands. Site-specific hybridization of these DNA-polymer chains on the single-stranded arms of the 3D-DNA scaffold gives efficient access to DNA-block copolymer cages. These biohybrid cages possess polymer chains that are programmably positioned in three dimensions on a DNA core and display increased nuclease resistance as compared to unfunctionalized DNA cages.

Original languageEnglish
Pages (from-to)4280-4286
Number of pages7
JournalJournal of the American Chemical Society
Volume134
Issue number9
DOIs
Publication statusPublished - 7 Mar 2012

    Fingerprint

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

McLaughlin, C. K., Hamblin, G. D., Hänni, K. D., Conway, J. W., Nayak, M. K., Carneiro, K. M. M., Bazzi, H. S., & Sleiman, H. F. (2012). Three-dimensional organization of block copolymers on "DNA- minimal" scaffolds. Journal of the American Chemical Society, 134(9), 4280-4286. https://doi.org/10.1021/ja210313p