Thioredoxin 1 enhances neovascularization and reduces ventricular remodeling during chronic myocardial infarction

A study using thioredoxin 1 transgenic mice

Ram Sudheer Adluri, Mahesh Thirunavukkarasu, Lijun Zhan, Yuzo Akita, Samson Mathews Samuel, Hajime Otani, Ye Shih Ho, Gautam Maulik, Nilanjana Maulik

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Oxidative stress plays a crucial role in disruption of neovascularization by alterations in thioredoxin 1 (Trx1) expression and its interaction with other proteins after myocardial infarction (MI). We previously showed that Trx1 has angiogenic properties, but the possible therapeutic significance of overexpressing Trx1 in chronic MI has not been elucidated. Therefore, we explored the angiogenic and cardioprotective potential of Trx1 in an in vivo MI model using transgenic mice overexpressing Trx1. Wild-type (W) and Trx1 transgenic (Trx1Tg/+) mice were randomized into W sham (WS), Trx1Tg/+ sham (TS), WMI, and TMI. MI was induced by permanent occlusion of LAD coronary artery. Hearts from mice overexpressing Trx1 exhibited reduced fibrosis and oxidative stress and attenuated cardiomyocyte apoptosis along with increased vessel formation compared to WMI. We found significant inhibition of Trx1 regulating proteins, TXNIP and AKAP 12, and increased p-Akt, p-eNOS, p-GSK-3β, HIF-1β, β-catenin, VEGF, Bcl-2, and survivin expression in TMI compared to WMI. Echocardiography performed 30days after MI revealed significant improvement in myocardial functions in TMI compared to WMI. Our study identifies a potential role for Trx1 overexpression and its association with its regulatory proteins TXNIP, AKAP12, and subsequent activation of Akt/GSK-3β/β-catenin/HIF-1β-mediated VEGF and eNOS expression in inducing angiogenesis and reduced ventricular remodeling. Hence, Trx1 and other proteins identified in our study may prove to be potential therapeutic targets in the treatment of ischemic heart disease.

Original languageEnglish
Pages (from-to)239-247
Number of pages9
JournalJournal of Molecular and Cellular Cardiology
Volume50
Issue number1
DOIs
Publication statusPublished - 1 Jan 2011
Externally publishedYes

Fingerprint

Thioredoxins
Ventricular Remodeling
Transgenic Mice
Myocardial Infarction
Glycogen Synthase Kinase 3
Catenins
Vascular Endothelial Growth Factor A
Proteins
Oxidative Stress
Cardiac Myocytes
Myocardial Ischemia
Echocardiography
Coronary Vessels
Fibrosis
Apoptosis

Keywords

  • Apoptosis
  • Myocardial infarction
  • Neovascularization
  • Oxidative stress
  • Thioredoxin 1
  • Ventricular remodeling

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Thioredoxin 1 enhances neovascularization and reduces ventricular remodeling during chronic myocardial infarction : A study using thioredoxin 1 transgenic mice. / Adluri, Ram Sudheer; Thirunavukkarasu, Mahesh; Zhan, Lijun; Akita, Yuzo; Mathews Samuel, Samson; Otani, Hajime; Ho, Ye Shih; Maulik, Gautam; Maulik, Nilanjana.

In: Journal of Molecular and Cellular Cardiology, Vol. 50, No. 1, 01.01.2011, p. 239-247.

Research output: Contribution to journalArticle

Adluri, Ram Sudheer ; Thirunavukkarasu, Mahesh ; Zhan, Lijun ; Akita, Yuzo ; Mathews Samuel, Samson ; Otani, Hajime ; Ho, Ye Shih ; Maulik, Gautam ; Maulik, Nilanjana. / Thioredoxin 1 enhances neovascularization and reduces ventricular remodeling during chronic myocardial infarction : A study using thioredoxin 1 transgenic mice. In: Journal of Molecular and Cellular Cardiology. 2011 ; Vol. 50, No. 1. pp. 239-247.
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