The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties

Jacques Bonnet, Ying Hui Wang, Gianpiero Spedale, R. Andrew Atkinson, Christophe Romier, Ali Hamiche, W. W.M.Pim Pijnappel, H. Th Marc Timmers, Lśszló Tora, Didier Devys, Bruno Kieffer

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

SAGA (Spt-Ada-Gcn5 acetyltransferase), a coactivator complex involved in chromatin remodelling, harbours both histone acetylation and deubiquitination activities. ATXN7/Sgf73 and ATXN7L3, two subunits of the SAGA deubiquitination module, contain an SCA7 domain characterized by an atypical zinc-finger. We show that the yeast Sgf73-SCA7 domain is not required to recruit Sgf73 into SAGA. Instead, it binds to nucleosomes, a property that is conserved in the human ATXN7-SCA7 domain but is lost in the ATXN7L3 domain. The solution structures of the SCA7 domain of both ATXN7 and ATXN7L3 reveal a new, common zinc-finger motif at the heart of two distinct folds, providing a molecular basis for the observed functional differences.

Original languageEnglish
Pages (from-to)612-618
Number of pages7
JournalEMBO Reports
Volume11
Issue number8
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

Fingerprint

Acetyltransferases
Nucleosomes
Plasticity
Zinc Fingers
Zinc
Acetylation
Chromatin Assembly and Disassembly
Ports and harbors
Histones
Yeast
Chromatin
Yeasts

Keywords

  • ATXN7
  • nucleosome
  • SAGA
  • ubiquitin
  • zinc-finger

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Bonnet, J., Wang, Y. H., Spedale, G., Atkinson, R. A., Romier, C., Hamiche, A., ... Kieffer, B. (2010). The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties. EMBO Reports, 11(8), 612-618. https://doi.org/10.1038/embor.2010.98

The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties. / Bonnet, Jacques; Wang, Ying Hui; Spedale, Gianpiero; Atkinson, R. Andrew; Romier, Christophe; Hamiche, Ali; Pijnappel, W. W.M.Pim; Timmers, H. Th Marc; Tora, Lśszló; Devys, Didier; Kieffer, Bruno.

In: EMBO Reports, Vol. 11, No. 8, 08.2010, p. 612-618.

Research output: Contribution to journalArticle

Bonnet, J, Wang, YH, Spedale, G, Atkinson, RA, Romier, C, Hamiche, A, Pijnappel, WWMP, Timmers, HTM, Tora, L, Devys, D & Kieffer, B 2010, 'The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties', EMBO Reports, vol. 11, no. 8, pp. 612-618. https://doi.org/10.1038/embor.2010.98
Bonnet, Jacques ; Wang, Ying Hui ; Spedale, Gianpiero ; Atkinson, R. Andrew ; Romier, Christophe ; Hamiche, Ali ; Pijnappel, W. W.M.Pim ; Timmers, H. Th Marc ; Tora, Lśszló ; Devys, Didier ; Kieffer, Bruno. / The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties. In: EMBO Reports. 2010 ; Vol. 11, No. 8. pp. 612-618.
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