The Ser96Ala variant in histidine-rich calcium-binding protein is associated with life-threatening ventricular arrhythmias in idiopathic dilated cardiomyopathy

Demetrios A. Arvanitis, Despina Sanoudou, Fotis Kolokathis, Elizabeth Vafiadaki, Vasiliki Papalouka, Aikaterini Kontrogianni-Konstantopoulos, George N. Theodorakis, Ioannis A. Paraskevaidis, Stamatios Adamopoulos, Gerald W. Dorn, Dimitrios Th Kremastinos, Evangelia G. Kranias

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    Abstract

    Aims: To investigate whether genetic variants of the histidine-rich calcium (HRC)-binding protein are associated with idiopathic dilated cardiomyopathy (DCM) and its progression. Methods and results: We screened 123 idiopathic DCM patients and 96 healthy individuals by single-strand conformation polymorphism analysis and direct sequencing for genetic variants in HRC. Six polymorphisms were detected: Leu35Leu (A/G), Ser43Asn (G/A), Ser96Ala (T/G), Glu202_Glu203insGlu (-/GAG), Asp261del (GAT/-), and an in-frame insertion of 51 amino acids at His321. The analysis of their frequencies did not reveal any significant correlation with DCM development. However, the Ser96Ala polymorphism exhibited a statistically significant correlation with the occurrence of life-threatening ventricular arrhythmias. During a follow-up of 4.02 ± 2.4 years, the risk for ventricular arrhythmias was higher (HR, 9.620; 95% CI, 2.183-42.394; P = 0.003) in the Ala/Ala patients, compared with Ser/Ser homozygous patients. On multivariable Cox regression analysis, the Ser96Ala polymorphism was the only significant genetic arrythmogenesis predictor in DCM patients (HR, 4.191; 95% CI, 0.838-20.967; P = 0.018). Conclusion: The Ser96Ala genetic variant of HRC is associated with life-threatening ventricular arrhythmias in idiopathic DCM and may serve as an independent predictor of susceptibility to arrhythmogenesis in the setting of DCM.

    Original languageEnglish
    Pages (from-to)2514-2525
    Number of pages12
    JournalEuropean Heart Journal
    Volume29
    Issue number20
    DOIs
    Publication statusPublished - 1 Oct 2008

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    Keywords

    • Calcium
    • Defibrillation
    • Prognosis
    • Sarcoplasmic reticulum

    ASJC Scopus subject areas

    • Cardiology and Cardiovascular Medicine

    Cite this

    Arvanitis, D. A., Sanoudou, D., Kolokathis, F., Vafiadaki, E., Papalouka, V., Kontrogianni-Konstantopoulos, A., Theodorakis, G. N., Paraskevaidis, I. A., Adamopoulos, S., Dorn, G. W., Kremastinos, D. T., & Kranias, E. G. (2008). The Ser96Ala variant in histidine-rich calcium-binding protein is associated with life-threatening ventricular arrhythmias in idiopathic dilated cardiomyopathy. European Heart Journal, 29(20), 2514-2525. https://doi.org/10.1093/eurheartj/ehn328