The roles of 3′-exoribonucleases and the exosome in trypanosome mRNA degradation

Abeer A. Fadda, Valentin Färber, Dorothea Droll, Christine Clayton

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The degradation of eukaryotic mRNAs can be initiated by deadenylation, decapping, or endonuclease cleavage. This is followed by 5′-3′ degradation by homologs of Xrn1, and/or 3′-5′ degradation by the exosome. We previously reported that, in African trypanosome Trypanosoma brucei, most mRNAs are deadenylated prior to degradation, and that depletion of the major 5′-3′ exoribonuclease XRNA preferentially stabilizes unstable mRNAs. We now show that depletion of either CAF1 or CNOT10, two components of the principal deadenylation complex, strongly inhibits degradation of most mRNAs. RNAi targeting another deadenylase, PAN2, or RRP45, a core component of the exosome, preferentially stabilized mRNAs with intermediate half-lives. RRP45 depletion resulted in a 5′ bias of mRNA sequences, suggesting action by a distributive 3′-5′ exoribonuclease. Results suggested that the exosome is involved in the processing of trypanosome snoRNAs. There was no correlation between effects on half-lives and on mRNA abundance.

Original languageEnglish
Pages (from-to)937-947
Number of pages11
JournalRNA
Volume19
Issue number7
DOIs
Publication statusPublished - Jul 2013
Externally publishedYes

Fingerprint

Exosomes
Trypanosomiasis
RNA Stability
Messenger RNA
Small Nucleolar RNA
Trypanosoma brucei brucei
Endonucleases
RNA Interference
poly A specific exoribonuclease

Keywords

  • CAF1
  • Deadenylation
  • Exosome
  • mRNA decay
  • mRNA degradation
  • PAN2
  • Trypanosoma

ASJC Scopus subject areas

  • Molecular Biology
  • Medicine(all)

Cite this

The roles of 3′-exoribonucleases and the exosome in trypanosome mRNA degradation. / Fadda, Abeer A.; Färber, Valentin; Droll, Dorothea; Clayton, Christine.

In: RNA, Vol. 19, No. 7, 07.2013, p. 937-947.

Research output: Contribution to journalArticle

Fadda, Abeer A. ; Färber, Valentin ; Droll, Dorothea ; Clayton, Christine. / The roles of 3′-exoribonucleases and the exosome in trypanosome mRNA degradation. In: RNA. 2013 ; Vol. 19, No. 7. pp. 937-947.
@article{18d39c81d4bf4078a395daf07ecb0df1,
title = "The roles of 3′-exoribonucleases and the exosome in trypanosome mRNA degradation",
abstract = "The degradation of eukaryotic mRNAs can be initiated by deadenylation, decapping, or endonuclease cleavage. This is followed by 5′-3′ degradation by homologs of Xrn1, and/or 3′-5′ degradation by the exosome. We previously reported that, in African trypanosome Trypanosoma brucei, most mRNAs are deadenylated prior to degradation, and that depletion of the major 5′-3′ exoribonuclease XRNA preferentially stabilizes unstable mRNAs. We now show that depletion of either CAF1 or CNOT10, two components of the principal deadenylation complex, strongly inhibits degradation of most mRNAs. RNAi targeting another deadenylase, PAN2, or RRP45, a core component of the exosome, preferentially stabilized mRNAs with intermediate half-lives. RRP45 depletion resulted in a 5′ bias of mRNA sequences, suggesting action by a distributive 3′-5′ exoribonuclease. Results suggested that the exosome is involved in the processing of trypanosome snoRNAs. There was no correlation between effects on half-lives and on mRNA abundance.",
keywords = "CAF1, Deadenylation, Exosome, mRNA decay, mRNA degradation, PAN2, Trypanosoma",
author = "Fadda, {Abeer A.} and Valentin F{\"a}rber and Dorothea Droll and Christine Clayton",
year = "2013",
month = "7",
doi = "10.1261/rna.038430.113",
language = "English",
volume = "19",
pages = "937--947",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "7",

}

TY - JOUR

T1 - The roles of 3′-exoribonucleases and the exosome in trypanosome mRNA degradation

AU - Fadda, Abeer A.

AU - Färber, Valentin

AU - Droll, Dorothea

AU - Clayton, Christine

PY - 2013/7

Y1 - 2013/7

N2 - The degradation of eukaryotic mRNAs can be initiated by deadenylation, decapping, or endonuclease cleavage. This is followed by 5′-3′ degradation by homologs of Xrn1, and/or 3′-5′ degradation by the exosome. We previously reported that, in African trypanosome Trypanosoma brucei, most mRNAs are deadenylated prior to degradation, and that depletion of the major 5′-3′ exoribonuclease XRNA preferentially stabilizes unstable mRNAs. We now show that depletion of either CAF1 or CNOT10, two components of the principal deadenylation complex, strongly inhibits degradation of most mRNAs. RNAi targeting another deadenylase, PAN2, or RRP45, a core component of the exosome, preferentially stabilized mRNAs with intermediate half-lives. RRP45 depletion resulted in a 5′ bias of mRNA sequences, suggesting action by a distributive 3′-5′ exoribonuclease. Results suggested that the exosome is involved in the processing of trypanosome snoRNAs. There was no correlation between effects on half-lives and on mRNA abundance.

AB - The degradation of eukaryotic mRNAs can be initiated by deadenylation, decapping, or endonuclease cleavage. This is followed by 5′-3′ degradation by homologs of Xrn1, and/or 3′-5′ degradation by the exosome. We previously reported that, in African trypanosome Trypanosoma brucei, most mRNAs are deadenylated prior to degradation, and that depletion of the major 5′-3′ exoribonuclease XRNA preferentially stabilizes unstable mRNAs. We now show that depletion of either CAF1 or CNOT10, two components of the principal deadenylation complex, strongly inhibits degradation of most mRNAs. RNAi targeting another deadenylase, PAN2, or RRP45, a core component of the exosome, preferentially stabilized mRNAs with intermediate half-lives. RRP45 depletion resulted in a 5′ bias of mRNA sequences, suggesting action by a distributive 3′-5′ exoribonuclease. Results suggested that the exosome is involved in the processing of trypanosome snoRNAs. There was no correlation between effects on half-lives and on mRNA abundance.

KW - CAF1

KW - Deadenylation

KW - Exosome

KW - mRNA decay

KW - mRNA degradation

KW - PAN2

KW - Trypanosoma

UR - http://www.scopus.com/inward/record.url?scp=84879289671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879289671&partnerID=8YFLogxK

U2 - 10.1261/rna.038430.113

DO - 10.1261/rna.038430.113

M3 - Article

C2 - 23697549

AN - SCOPUS:84879289671

VL - 19

SP - 937

EP - 947

JO - RNA

JF - RNA

SN - 1355-8382

IS - 7

ER -