The role of monoubiquitination in endocytic degradation of human ether-a-go-go-related gene (hERG) channels under low K+ conditions

Tao Sun, Jun Guo, Heidi Shallow, Tonghua Yang, Jianmin Xu, Wentao Li, Christian Hanson, James G. Wu, Xian Li, Hamid Massaeli, Shetuan Zhang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A reduction in extracellular K+ concentration ([K+]o) causes cardiac arrhythmias and triggers internalization of the cardiac rapidly activating delayed rectifier potassium channel (IKr) encoded by the human ether-a-go-go-related gene (hERG). We investigated the role of ubiquitin (Ub) in endocytic degradation of hERG channels stably expressed in HEK cells. Under low K+ conditions, UbKO, a lysine-less mutant Ub that only supports monoubiquitination, preferentially interacted and selectively enhanced degradation of the mature hERG channels. Overexpression of Vps24 protein, also known as charged multivesicular body protein 3, significantly accelerated degradation of mature hERG channels, whereas knockdown of Vps24 impeded this process. Moreover, the lysosomal inhibitor bafilomycin A1 inhibited degradation of the internalized mature hERG channels. Thus, monoubiquitination directs mature hERG channels to degrade through the multivesicular body/lysosome pathway. Interestingly, the protease inhibitor lactacystin inhibited the low K+-induced hERG endocytosis and concomitantly led to an accumulation of monoubiquitinated mature hERG channels, suggesting that deubiquitination is also required for the endocytic degradation. Consistently, overexpression of the endosomal deubiquitinating enzyme signal transducing adaptor molecule-binding protein significantly accelerated whereas knockdown of endogenous signal transducing adaptor molecule-binding protein impeded degradation of the mature hERG channels under low K+ conditions. Thus, monoubiquitin dynamically mediates endocytic degradation of mature hERG channels under low K+ conditions.

Original languageEnglish
Pages (from-to)6751-6759
Number of pages9
JournalJournal of Biological Chemistry
Volume286
Issue number8
DOIs
Publication statusPublished - 25 Feb 2011
Externally publishedYes

Fingerprint

Ether
Genes
Degradation
Multivesicular Bodies
Ubiquitin
Delayed Rectifier Potassium Channels
Carrier Proteins
Molecules
Corrosion inhibitors
Endocytosis
Lysosomes
Protease Inhibitors
Proteolysis
Lysine
Cardiac Arrhythmias
Proteins
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The role of monoubiquitination in endocytic degradation of human ether-a-go-go-related gene (hERG) channels under low K+ conditions. / Sun, Tao; Guo, Jun; Shallow, Heidi; Yang, Tonghua; Xu, Jianmin; Li, Wentao; Hanson, Christian; Wu, James G.; Li, Xian; Massaeli, Hamid; Zhang, Shetuan.

In: Journal of Biological Chemistry, Vol. 286, No. 8, 25.02.2011, p. 6751-6759.

Research output: Contribution to journalArticle

Sun, Tao ; Guo, Jun ; Shallow, Heidi ; Yang, Tonghua ; Xu, Jianmin ; Li, Wentao ; Hanson, Christian ; Wu, James G. ; Li, Xian ; Massaeli, Hamid ; Zhang, Shetuan. / The role of monoubiquitination in endocytic degradation of human ether-a-go-go-related gene (hERG) channels under low K+ conditions. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 8. pp. 6751-6759.
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