The role of interleukin-6 in mitogenic T-cell activation: Detection of interleukin-2 heteronuclear RNA by polymerase chain reaction

Gerd Walz, Christopher Stevens, Bernd Zanker, Larry B. Melton, Steven C. Clark, Manikkam Suthanthiran, Terry B. Strom

Research output: Contribution to journalArticle

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Abstract

It has been documented that interleukin-6 (IL-6) supports the proliferation of purified, anti-C133-stimulated murine T cells. We found that stimulation of human peripheral blood mononuclear cells (PBMCs) with anti-CD3 induced a significant accumulation of IL-6 mRNA, indicating that antigen-mediated T-cell activation may involve IL-6 release from accessory cells. Phytohemagglutinin (PHA) had little effect upon IL-6 gene expression. In keeping with these findings, anti-IL-6 reduced but did not abolish anti-CD3-mediated proliferation of PBMCs, but had no significant effect upon PHA-stimulated proliferation. The addition of recombinant (r) IL-6 enhanced the proliferation of anti-CD3-stimulated PBMCs and increased the accumulation of IL-2 mRNA in PHA-stimulated PBMCs during the first 5 hr of culture. Nuclear run-off experiments did not reveal significant changes in IL-2 transcription in PHA plus rIL-6-treated PBMCs attempting to assume that IL-6 mediates stabilization of IL-2 mRNA. However, monitoring of partially spliced IL-2 mRNA by polymerase chain reaction revealed a clear increase in IL-2 heteronuclear RNA. Thus IL-6 increases the rate of IL-2 transcription which was not detectable by conventional in vitro transcription assays. We conclude that anti-CD3 triggers T-cell proliferation through a process that is partially but not entirely dependent upon release of IL-6. IL-6, in turn, supports IL-2 transcription. Insofar as anti-CD3 mimicks antigen-triggered activation of the T-cell receptor complex, IL-6 appears to support the early immune response by augmenting antigen-triggered IL-2 gene expression.

Original languageEnglish
Pages (from-to)511-519
Number of pages9
JournalCellular Immunology
Volume134
Issue number2
DOIs
Publication statusPublished - 1 Jan 1991
Externally publishedYes

Fingerprint

DNA-Directed RNA Polymerases
Interleukin-2
Interleukin-6
T-Lymphocytes
Polymerase Chain Reaction
Phytohemagglutinins
Blood Cells
Messenger RNA
CD27 Antigens
CD3 Antigens
Gene Expression
Histocompatibility Antigens Class II
T-Cell Antigen Receptor
Cell Proliferation
RNA

ASJC Scopus subject areas

  • Immunology

Cite this

The role of interleukin-6 in mitogenic T-cell activation : Detection of interleukin-2 heteronuclear RNA by polymerase chain reaction. / Walz, Gerd; Stevens, Christopher; Zanker, Bernd; Melton, Larry B.; Clark, Steven C.; Suthanthiran, Manikkam; Strom, Terry B.

In: Cellular Immunology, Vol. 134, No. 2, 01.01.1991, p. 511-519.

Research output: Contribution to journalArticle

Walz, Gerd ; Stevens, Christopher ; Zanker, Bernd ; Melton, Larry B. ; Clark, Steven C. ; Suthanthiran, Manikkam ; Strom, Terry B. / The role of interleukin-6 in mitogenic T-cell activation : Detection of interleukin-2 heteronuclear RNA by polymerase chain reaction. In: Cellular Immunology. 1991 ; Vol. 134, No. 2. pp. 511-519.
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