The role of histone H1 and non-structured domains of core histones in maintaining the orientation of nucleosomes within the chromatin fiber

Vladimir L. Makarov, Stephan I. Dimitrov, Irina R. Tsaneva, Iliya G. Pashev

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Calf thymus chromatin was digested with trypsin and the structural alterations which occured were followed by flow linear dichroism. After a sharp initial increase, the amplitude of the positive signal gradually decreased followed by a change of the sign of the dichroism and further increase of the negative signal up to a plateau. These changes of the dichroism were compared to the respective changes in the histone pattern. It was shown that the positive dichroism of chromatin did not depend on the condensation state of chromatin, and that the orientation of the nucleosomes along the chromatin fiber was maintained by the globular domain of H1 and the non-structured parts of core histones.

Original languageEnglish
Pages (from-to)1021-1027
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume122
Issue number3
DOIs
Publication statusPublished - 16 Aug 1984
Externally publishedYes

Fingerprint

Nucleosomes
Histones
Chromatin
Fibers
Thymus
Trypsin
Thymus Gland
Condensation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The role of histone H1 and non-structured domains of core histones in maintaining the orientation of nucleosomes within the chromatin fiber. / Makarov, Vladimir L.; Dimitrov, Stephan I.; Tsaneva, Irina R.; Pashev, Iliya G.

In: Biochemical and Biophysical Research Communications, Vol. 122, No. 3, 16.08.1984, p. 1021-1027.

Research output: Contribution to journalArticle

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