The RNA-binding protein HuR promotes cell migration and cell invasion by stabilizing the β-actin mRNA in a U-rich-element-dependent manner

Virginie Dormoy-Raclet, Isabelle Ménard, Eveline Clair, Ghada Kurban, Rachid Mazroui, Sergio Di Marco, Christopher Von Roretz, Arnim Pause, Imed Gallouzi

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

A high expression level of the β-actin protein is required for important biological mechanisms, such as maintaining cell shape, growth, and motility. Although the elevated cellular level of the β-actin protein is directly linked to the long half-life of its mRNA, the molecular mechanisms responsible for this effect are unknown. Here we show that the RNA-binding protein HuR stabilizes the β-actin mRNA by associating with a uridine-rich element within its 3′ untranslated region. Using RNA interference to knock down the expression of HuR in HeLa cells, we demonstrate that HuR plays an important role in the stabilization but not in the nuclear/cytoplasmic distribution of the β-actin mRNA. HuR depletion in HeLa cells alters key β-actin-based cytoskeleton functions, such as cell adhesion, migration, and invasion, and these defects correlate with a loss of the actin stress fiber network. Together our data establish that the posttranscriptional event involving HuR-mediated β-actin mRNA stabilization could be a part of the regulatory mechanisms responsible for maintaining cell integrity, which is a prerequisite for avoiding transformation and tumor formation.

Original languageEnglish
Pages (from-to)5365-5380
Number of pages16
JournalMolecular and Cellular Biology
Volume27
Issue number15
DOIs
Publication statusPublished - Aug 2007
Externally publishedYes

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RNA-Binding Proteins
Cell Movement
Actins
Messenger RNA
HeLa Cells
Stress Fibers
Cell Shape
Uridine
3' Untranslated Regions
RNA Interference
Actin Cytoskeleton
Cell Adhesion
Half-Life
Proteins
Growth
Neoplasms

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The RNA-binding protein HuR promotes cell migration and cell invasion by stabilizing the β-actin mRNA in a U-rich-element-dependent manner. / Dormoy-Raclet, Virginie; Ménard, Isabelle; Clair, Eveline; Kurban, Ghada; Mazroui, Rachid; Di Marco, Sergio; Von Roretz, Christopher; Pause, Arnim; Gallouzi, Imed.

In: Molecular and Cellular Biology, Vol. 27, No. 15, 08.2007, p. 5365-5380.

Research output: Contribution to journalArticle

Dormoy-Raclet, Virginie ; Ménard, Isabelle ; Clair, Eveline ; Kurban, Ghada ; Mazroui, Rachid ; Di Marco, Sergio ; Von Roretz, Christopher ; Pause, Arnim ; Gallouzi, Imed. / The RNA-binding protein HuR promotes cell migration and cell invasion by stabilizing the β-actin mRNA in a U-rich-element-dependent manner. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 15. pp. 5365-5380.
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