The Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on membrane currents and intrasynaptosomal calcium

K. P. Mohanakumar, S. Samanta Ray, Dietrich Busselberg

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Effects of the neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its metabolite, 1-methyl-4-phenyl pyridinium (MPP+) were investigated on neuronal membrane currents and intrasynaptosomal calcium levels employing whole cell patch clamp and FURA-2AM respectively. Membrane currents elicited by voltage ramps as well as voltage activated potassium-currents were studied in the cultured rat dorsal root ganglia (DRG) neurons. These neurons contained 0.36 ± 0.04 pmol/mg of dopamine as detected employing HPLC-electrochemistry. MPTP and MPP+ (1μM) respectively caused significant increase (25 ± 4%) or inhibition (53 ± 10%) of the total membrane current. Potassium-currents elicited by voltage jumps from -80 to 0 mV were significantly inhibited (68 ± 8%) by MPP+, but MPTP caused no effect. MP+-induced increase in the FURA-2 fluorescence signal was insensitive to extrasynaptosomal calcium, indicated intrasynaptosomal mobilization by the neurotoxin. These results suggest involvement of ion channels in MPTP neurotoxicity, and imply its role in the etiopathology of Parkinson's disease.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalNeuroscience Research Communications
Volume30
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

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1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Neurotoxins
Calcium
Membranes
Potassium
1-Methyl-4-phenylpyridinium
Electrochemistry
Neurons
Architectural Accessibility
Spinal Ganglia
Ion Channels
Parkinson Disease
Dopamine
Fluorescence
High Pressure Liquid Chromatography
4-phenyl-1,2,3,6-tetrahydropyridine

Keywords

  • Dopamine, MPP
  • Neurotoxicity
  • Patch-clamp studies
  • Voltage activated K-currents

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on membrane currents and intrasynaptosomal calcium. / Mohanakumar, K. P.; Samanta Ray, S.; Busselberg, Dietrich.

In: Neuroscience Research Communications, Vol. 30, No. 1, 2002, p. 35-42.

Research output: Contribution to journalArticle

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