The Olfactory Transcriptomes of Mice

Ximena Ibarra-Soria, Maria O. Levitin, Luis Miguel Rodrigues Saraiva, Darren W. Logan

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The olfactory (OR) and vomeronasal receptor (VR) repertoires are collectively encoded by 1700 genes and pseudogenes in the mouse genome. Most OR and VR genes were identified by comparative genomic techniques and therefore, in many of those cases, only their protein coding sequences are defined. Some also lack experimental support, due in part to the similarity between them and their monogenic, cell-specific expression in olfactory tissues. Here we use deep RNA sequencing, expression microarray and quantitative RT-PCR in both the vomeronasal organ and whole olfactory mucosa to quantify their full transcriptomes in multiple male and female mice. We find evidence of expression for all VR, and almost all OR genes that are annotated as functional in the reference genome, and use the data to generate over 1100 new, multi-exonic, significantly extended receptor gene annotations. We find that OR and VR genes are neither equally nor randomly expressed, but have reproducible distributions of abundance in both tissues. The olfactory transcriptomes are only minimally different between males and females, suggesting altered gene expression at the periphery is unlikely to underpin the striking sexual dimorphism in olfactory-mediated behavior. Finally, we present evidence that hundreds of novel, putatively protein-coding genes are expressed in these highly specialized olfactory tissues, and carry out a proof-of-principle validation. Taken together, these data provide a comprehensive, quantitative catalog of the genes that mediate olfactory perception and pheromone-evoked behavior at the periphery.

Original languageEnglish
JournalPLoS Genetics
Volume10
Issue number9
DOIs
Publication statusPublished - 1 Sep 2014
Externally publishedYes

Fingerprint

Transcriptome
transcriptome
Odorant Receptors
gene
mice
Genes
receptors
genes
Olfactory Perception
Vomeronasal Organ
Genome
RNA Sequence Analysis
Molecular Sequence Annotation
High-Throughput Nucleotide Sequencing
Olfactory Mucosa
Pseudogenes
Pheromones
genome
Sex Characteristics
protein

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

The Olfactory Transcriptomes of Mice. / Ibarra-Soria, Ximena; Levitin, Maria O.; Rodrigues Saraiva, Luis Miguel; Logan, Darren W.

In: PLoS Genetics, Vol. 10, No. 9, 01.09.2014.

Research output: Contribution to journalArticle

Ibarra-Soria, Ximena ; Levitin, Maria O. ; Rodrigues Saraiva, Luis Miguel ; Logan, Darren W. / The Olfactory Transcriptomes of Mice. In: PLoS Genetics. 2014 ; Vol. 10, No. 9.
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