The N-terminus of histone H2B, but not that of histone H3 or its phosphorylation, is essential for chromosome condensation

Anne Elisabeth De La Barre, Dimitri Angelov, Annie Molla, Stefan Dimitrov

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

We have studied the role of individual histone N-termini and the phosphorylation of histone H3 in chromosome condensation. Nucleosomes, reconstituted with histone octamers containing different combinations of recombinant full-length and tailless histones, were used as competitors for chromosome assembly in Xenopus egg extracts. Nucleosomes reconstituted with intact octamers inhibited chromosome condensation as efficiently as the native ones, while tailless nucleosomes were unable to affect this process. Importantly, the addition to the extract of particles containing only intact histone H2B strongly interfered with chromosome formation while such an effect was not observed with particles lacking the N-terminal tail of H2B. This demonstrates that the inhibition effect observed in the presence of competitor nucleosomes is mainly due to the N-terminus of this histone, which, therefore, is essential for chromosome condensation. Nucleosomes in which all histones but H3 were tailless did not impede chromosome formation. In addition, when competitor nucleosome particles were reconstituted with full-length H2A, H2B and H4 and histone H3 mutated at the phosphorylable serine 10 or serine 28, their inhibiting efficiency was identical to that of the native particles. Hence, the tail of H3, whether intact or phosphorylated, is not important for chromosome condensation. A novel hypothesis, termed 'the ready production label' was suggested to explain the role of histone H3 phosphorylation during cell division.

Original languageEnglish
Pages (from-to)6383-6393
Number of pages11
JournalEMBO Journal
Volume20
Issue number22
DOIs
Publication statusPublished - 15 Nov 2001
Externally publishedYes

Fingerprint

Phosphorylation
Chromosomes
Histones
Condensation
Nucleosomes
Serine
Tail
Xenopus
Cell Division
Ovum
Labels
Cells

Keywords

  • Chromosome
  • Condensation
  • H3 phosphorylation
  • Histone H2B
  • Histone tails

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The N-terminus of histone H2B, but not that of histone H3 or its phosphorylation, is essential for chromosome condensation. / De La Barre, Anne Elisabeth; Angelov, Dimitri; Molla, Annie; Dimitrov, Stefan.

In: EMBO Journal, Vol. 20, No. 22, 15.11.2001, p. 6383-6393.

Research output: Contribution to journalArticle

De La Barre, Anne Elisabeth ; Angelov, Dimitri ; Molla, Annie ; Dimitrov, Stefan. / The N-terminus of histone H2B, but not that of histone H3 or its phosphorylation, is essential for chromosome condensation. In: EMBO Journal. 2001 ; Vol. 20, No. 22. pp. 6383-6393.
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