The Mitotic Chromosome Is an Assembly of Rigid Elastic Axes Organized by Structural Maintenance of Chromosomes (SMC) Proteins and Surrounded by a Soft Chromatin Envelope

Sébastien Almagro, Daniel Riveline, Tatsuya Hirano, Bahram Houchmandzadeh, Stefan Dimitrov

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The structure of mitotic chromosomes is still poorly understood. Here we describe the use of a novel approach based on elasticity measurements of a single chromosome for studying the organization of these objects. The data reveal that mitotic chromosomes exhibit a non-homogenous structure consisting of rigid elastic axes surrounded by a soft chromatin envelope. The chemical continuity of DNA, but not RNA, was required for the maintenance of these axes. The axes show a modular structure, and the structural maintenance of chromosomes (SMC) proteins participate in their organization. Topoisomerase II was not involved in either the organization of the axes or the maintenance of the mitotic chromosomes. A model for the assembly and the structure of the mitotic chromosome is proposed. According this model, the chromosome axes are dynamic structures that assemble at the onset and disassemble the end of mitosis, respectively. The SMC proteins, in addition to maintaining axis elasticity, are essential for the determination of the rod-like chromosome shape. The extreme compaction of mitotic chromosomes is determined mainly by the high amount of bivalent ions bound to DNA at mitosis.

Original languageEnglish
Pages (from-to)5118-5126
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number7
DOIs
Publication statusPublished - 13 Feb 2004
Externally publishedYes

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Chromosomes
Chromatin
Maintenance
Proteins
Chromosome Structures
Elasticity
Mitosis
Type II DNA Topoisomerase
DNA
RNA
Ions
Compaction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The Mitotic Chromosome Is an Assembly of Rigid Elastic Axes Organized by Structural Maintenance of Chromosomes (SMC) Proteins and Surrounded by a Soft Chromatin Envelope. / Almagro, Sébastien; Riveline, Daniel; Hirano, Tatsuya; Houchmandzadeh, Bahram; Dimitrov, Stefan.

In: Journal of Biological Chemistry, Vol. 279, No. 7, 13.02.2004, p. 5118-5126.

Research output: Contribution to journalArticle

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