The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility

Alexandra K. Marr, Joerg Overhage, Manjeet Bains, Robert E W Hancock

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is an important nosocomial opportunistic human pathogen and a major cause of chronic lung infections in individuals with cystic fibrosis. Serious infections by this organism are often treated with a combination of aminoglycosides and semi-synthetic penicillins. Subinhibitory concentrations of antibiotics are now being recognized for their role in microbial persistence and the development of antimicrobial resistance, two very important clinical phenomena. An extensive screen of a P. aeruginosa PAO1 luciferase gene fusion library was performed to identify genes that were differentially regulated during exposure to subinhibitory gentamicin. It was demonstrated that subinhibitory concentrations of gentamicin and tobramycin induced a set of genes that are likely to affect the interaction of P. aeruginosa with host cells, including the gene encoding Lon protease, which is known to play a major role in protein quality control. Studies with a Ion mutant compared to its parent and a complemented strain indicated that this protein was essential for biofilm formation and motility in P. aeruginosa.

Original languageEnglish
Pages (from-to)474-482
Number of pages9
JournalMicrobiology
Volume153
Issue number2
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

Fingerprint

Protease La
Aminoglycosides
Biofilms
Pseudomonas aeruginosa
Gentamicins
Genes
Tobramycin
Gene Fusion
Infection
Luciferases
Gene Library
Cystic Fibrosis
Penicillins
Quality Control
Proteins
Ions
Anti-Bacterial Agents
Lung

ASJC Scopus subject areas

  • Microbiology

Cite this

The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility. / Marr, Alexandra K.; Overhage, Joerg; Bains, Manjeet; Hancock, Robert E W.

In: Microbiology, Vol. 153, No. 2, 02.2007, p. 474-482.

Research output: Contribution to journalArticle

Marr, Alexandra K. ; Overhage, Joerg ; Bains, Manjeet ; Hancock, Robert E W. / The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility. In: Microbiology. 2007 ; Vol. 153, No. 2. pp. 474-482.
@article{8181b849fd7647668a4d081f4b8b4e66,
title = "The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility",
abstract = "Pseudomonas aeruginosa is an important nosocomial opportunistic human pathogen and a major cause of chronic lung infections in individuals with cystic fibrosis. Serious infections by this organism are often treated with a combination of aminoglycosides and semi-synthetic penicillins. Subinhibitory concentrations of antibiotics are now being recognized for their role in microbial persistence and the development of antimicrobial resistance, two very important clinical phenomena. An extensive screen of a P. aeruginosa PAO1 luciferase gene fusion library was performed to identify genes that were differentially regulated during exposure to subinhibitory gentamicin. It was demonstrated that subinhibitory concentrations of gentamicin and tobramycin induced a set of genes that are likely to affect the interaction of P. aeruginosa with host cells, including the gene encoding Lon protease, which is known to play a major role in protein quality control. Studies with a Ion mutant compared to its parent and a complemented strain indicated that this protein was essential for biofilm formation and motility in P. aeruginosa.",
author = "Marr, {Alexandra K.} and Joerg Overhage and Manjeet Bains and Hancock, {Robert E W}",
year = "2007",
month = "2",
doi = "10.1099/mic.0.2006/002519-0",
language = "English",
volume = "153",
pages = "474--482",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "2",

}

TY - JOUR

T1 - The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility

AU - Marr, Alexandra K.

AU - Overhage, Joerg

AU - Bains, Manjeet

AU - Hancock, Robert E W

PY - 2007/2

Y1 - 2007/2

N2 - Pseudomonas aeruginosa is an important nosocomial opportunistic human pathogen and a major cause of chronic lung infections in individuals with cystic fibrosis. Serious infections by this organism are often treated with a combination of aminoglycosides and semi-synthetic penicillins. Subinhibitory concentrations of antibiotics are now being recognized for their role in microbial persistence and the development of antimicrobial resistance, two very important clinical phenomena. An extensive screen of a P. aeruginosa PAO1 luciferase gene fusion library was performed to identify genes that were differentially regulated during exposure to subinhibitory gentamicin. It was demonstrated that subinhibitory concentrations of gentamicin and tobramycin induced a set of genes that are likely to affect the interaction of P. aeruginosa with host cells, including the gene encoding Lon protease, which is known to play a major role in protein quality control. Studies with a Ion mutant compared to its parent and a complemented strain indicated that this protein was essential for biofilm formation and motility in P. aeruginosa.

AB - Pseudomonas aeruginosa is an important nosocomial opportunistic human pathogen and a major cause of chronic lung infections in individuals with cystic fibrosis. Serious infections by this organism are often treated with a combination of aminoglycosides and semi-synthetic penicillins. Subinhibitory concentrations of antibiotics are now being recognized for their role in microbial persistence and the development of antimicrobial resistance, two very important clinical phenomena. An extensive screen of a P. aeruginosa PAO1 luciferase gene fusion library was performed to identify genes that were differentially regulated during exposure to subinhibitory gentamicin. It was demonstrated that subinhibitory concentrations of gentamicin and tobramycin induced a set of genes that are likely to affect the interaction of P. aeruginosa with host cells, including the gene encoding Lon protease, which is known to play a major role in protein quality control. Studies with a Ion mutant compared to its parent and a complemented strain indicated that this protein was essential for biofilm formation and motility in P. aeruginosa.

UR - http://www.scopus.com/inward/record.url?scp=33847218319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847218319&partnerID=8YFLogxK

U2 - 10.1099/mic.0.2006/002519-0

DO - 10.1099/mic.0.2006/002519-0

M3 - Article

VL - 153

SP - 474

EP - 482

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 2

ER -