The influence of muscle type and dystrophin deficiency on murine expression profiles

Judith N. Haslett, Peter B. Kang, Mei Han, Alvin T. Kho, Despina Sanoudou, Jay M. Volinski, Alan H. Beggs, Isaac S. Kohane, Louis M. Kunkel

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The phenotypic differences among Duchenne muscular dystrophy patients, max mice, and mdx5cv mice suggest that despite the common etiology of dystrophin deficiency, secondary mechanisms have a substantial influence on phenotypic severity. The differential response of various skeletal muscles to dystrophin deficiency supports this hypothesis. To explore these differences, gene expression profiles were generated from duplicate RNA targets extracted from six different skeletal muscles (diaphragm, soleus, gastrocnemius, quadriceps, tibialis anterior, and extensor digitorum longus) from wildtype, max, and mdx5cv mice, resulting in 36 data sets for 18 muscle samples. The data sets were compared in three different ways: (1) among wild-type samples only, (2) among all 36 data sets, and (3) between strains for each muscle type. The molecular profiles of soleus and diaphragm separate significantly from the other four muscle types and from each other. Fiber-type proportions can explain some of these differences. These variations in wild-type gene expression profiles may also reflect biomechanical differences known to exist among skeletal muscles. Further exploration of the genes that most distinguish these muscles may help explain the origins of the biomechanical differences and the reasons why some muscles are more resistant than others to dystrophin deficiency.

Original languageEnglish
Pages (from-to)739-748
Number of pages10
JournalMammalian Genome
Volume16
Issue number10
DOIs
Publication statusPublished - 1 Oct 2005
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

Cite this

Haslett, J. N., Kang, P. B., Han, M., Kho, A. T., Sanoudou, D., Volinski, J. M., Beggs, A. H., Kohane, I. S., & Kunkel, L. M. (2005). The influence of muscle type and dystrophin deficiency on murine expression profiles. Mammalian Genome, 16(10), 739-748. https://doi.org/10.1007/s00335-005-0053-8