The incorporation of the novel histone variant H2AL2 confers unusual structural and functional properties of the nucleosome

Sajad Hussain Syed, Mathieu Boulard, Manu Shubhdarshan Shukla, Thierry Gautier, Andrew Travers, Jan Bednar, Cendrine Faivre-Moskalenko, Stefan Dimitrov, Dimitar Angelov

Research output: Contribution to journalArticle

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Abstract

In this work we have studied the properties of the novel mouse histone variant H2AL2. H2AL2 was used to reconstitute nucleosomes and the structural and functional properties of these particles were studied by a combination of biochemical approaches, atomic force microscopy (AFM) and electron cryo-microscopy. DNase I and hydroxyl radical footprinting as well as micrococcal and exonuclease III digestion demonstrated an altered structure of the H2AL2 nucleosomes all over the nucleosomal DNA length. Restriction nuclease accessibility experiments revealed that the interactions of the H2AL2 histone octamer with the ends of the nucleosomal DNA are highly perturbed. AFM imaging showed that the H2AL2 histone octamer was complexed with only ∼130 bp of DNA. H2AL2 reconstituted trinucleosomes exhibited a type of a 'beads on a string' structure, which was quite different from the equilateral triangle 3D organization of conventional H2A trinucleosomes. The presence of H2AL2 affected both the RSC and SWI/SNF remodeling and mobilization of the variant particles. These unusual properties of the H2AL2 nucleosomes suggest a specific role of H2AL2 during mouse spermiogenesis.

Original languageEnglish
Pages (from-to)4684-4695
Number of pages12
JournalNucleic Acids Research
Volume37
Issue number14
DOIs
Publication statusPublished - 2009
Externally publishedYes

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Nucleosomes
Histones
Atomic Force Microscopy
DNA
Cryoelectron Microscopy
Deoxyribonuclease I
Spermatogenesis
Hydroxyl Radical
Digestion

ASJC Scopus subject areas

  • Genetics

Cite this

Syed, S. H., Boulard, M., Shukla, M. S., Gautier, T., Travers, A., Bednar, J., ... Angelov, D. (2009). The incorporation of the novel histone variant H2AL2 confers unusual structural and functional properties of the nucleosome. Nucleic Acids Research, 37(14), 4684-4695. https://doi.org/10.1093/nar/gkp473

The incorporation of the novel histone variant H2AL2 confers unusual structural and functional properties of the nucleosome. / Syed, Sajad Hussain; Boulard, Mathieu; Shukla, Manu Shubhdarshan; Gautier, Thierry; Travers, Andrew; Bednar, Jan; Faivre-Moskalenko, Cendrine; Dimitrov, Stefan; Angelov, Dimitar.

In: Nucleic Acids Research, Vol. 37, No. 14, 2009, p. 4684-4695.

Research output: Contribution to journalArticle

Syed, SH, Boulard, M, Shukla, MS, Gautier, T, Travers, A, Bednar, J, Faivre-Moskalenko, C, Dimitrov, S & Angelov, D 2009, 'The incorporation of the novel histone variant H2AL2 confers unusual structural and functional properties of the nucleosome', Nucleic Acids Research, vol. 37, no. 14, pp. 4684-4695. https://doi.org/10.1093/nar/gkp473
Syed, Sajad Hussain ; Boulard, Mathieu ; Shukla, Manu Shubhdarshan ; Gautier, Thierry ; Travers, Andrew ; Bednar, Jan ; Faivre-Moskalenko, Cendrine ; Dimitrov, Stefan ; Angelov, Dimitar. / The incorporation of the novel histone variant H2AL2 confers unusual structural and functional properties of the nucleosome. In: Nucleic Acids Research. 2009 ; Vol. 37, No. 14. pp. 4684-4695.
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