The human intersectin genes and their spliced variants are differentially expressed

Carles Pucharcos, Caty Casas, Marga Nadal, Xavier P. Estivill, Susana De La Luna

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Human intersectins (ITSN1 and ITSN2) are members of a conserved family of proteins involved in clathrin-mediated endocytosis. A short and a long isoform with different protein domain compositions have been described for both human intersectins. Here, we have resolved the exon/intron structure of the ITSN2 gene to explain the genomic origin of its alternatively spliced transcripts. Comparison of the two ITSN human genes shows a high level of conservation in their genomic organization, including the main alternative splicing events. An extensive tissue expression analysis of the two predominant transcripts as well as other minor variants shows that ITSN expression is under tissue and developmental controls. Their differential expression is made more evident when the expression of both intersectins is studied by in situ hybridization in mouse brain.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1521
Issue number1-3
DOIs
Publication statusPublished - 31 Oct 2001
Externally publishedYes

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Keywords

  • Alternative splicing
  • Clathrin
  • Endocytosis
  • Gene structure
  • In situ hybridization
  • Intersectin

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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